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文献和实验v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
7.6, and 1.37 MNaCl. Store at room temperature. 2. TBS with Tween (TBS-T) (10×): prepare a TBS solution (X1)and add 0.1% (v/v) Tween-20. 3. 5% (w/v) Non-fat dry milk in TBS-T. 4. Primary antibody: APC (Ab-1) antibody from OncogeneResearch.
‐controlled mechanism for carbon depletion. J. Appl. Phys. 106:013311. Gomez, E.W., Clack, N.G., Wu, H.J., and Groves, J.T. 2009. Like‐charge interactions between colloidal particles are asymmetric with respect to sign. Soft Matter 5:1931‐1936.
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
2 at a sufficiently low level to be used for an interactor hunt. However, if one were to then attempt to thoroughly screen a library of 3 x 106 individual cDNAs by plating over 3 x 106 CFU onto the leu- selection plates, at least 3000 colonies would form
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