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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Lyophilized or Liquid
- 保存条件:
-20°C
- 克隆性:
Polyclonal
- 宿主:
Rabbit
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 抗体名:
Cy3标记的R-钙粘附分子抗体
- 规格:
100ul
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文献和实验) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
Large-scale immunofluorescence
) or orbital platform shaker (240 r.p.m.). Centrifuge microtiter dishes for 5 minutes at 500 X g to pellet cells. Fix cells by adding 1/10 volume of 37% formaldehyde and incubating at room temperature for 15 minutes to 1 hour 2 without shaking
) or orbital platform shaker (240 r.p.m.). Centrifuge microtiter dishes for 5 minutes at 500 X g to pellet cells. Fix cells by adding 1/10 volume of 37 formaldehyde and incubating at room temperature for 15 minutes to 1 hour 2 without shaking.
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