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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm Newport 10Q20HE.2 4 SUPREMA Optical Mount, 1.0 inch diameter
For 1 ml of digestion buffer: 60 µl 5M NaCl 10 µl 3M sodium acetate, pH 5.5 20 µl 0.1M zinc sulfate 2.5 µl S1 nuclease (400 U/µl) 907.5 µl water 7. Add 200 µl of S1 digestion buffer to sample. 8. Incubate at 37 deg C for 45 minutes. 9. Add 1 µl
sulfate2.5 µl S1 nuclease (400 U/µl)907.5 µl water7. Add 200 µl of S1 digestion buffer to sample.8. Incubate at 37 deg C for 45 minutes.9. Add 1 µl glycogen (20 mg/ml), 500 µl EtOH and precipitate on ice for 15 minutes.10. Spin for 30 minutes at 4 deg C.11
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