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文献和实验One of the major challenges in human B cell immunology field has been the objective to establish stable monoclonal cells lines that express the B cell receptor (BCR) on their cell surface and secrete antibodies. Such a system is extremely
Isolation of Untouched Human B Cells by Depletion of Non-B Cells
-bound cells with a magnet. Discard the bead-bound cells and use the remaining, untouched human B cells for any application. 实验试剂 Mixer allowing both tilting and rotation. Magnets Isolation Buffer: Ca2 and Mg2 free
Expression Cloning of Human B Cell Immunoglobulins
about the specificity of the antibodies expressed by lymphoma cells, and the role of antibody-specificity in lymphomagenesis remains elusive. Here, we describe a strategy to characterize the antibody reactivity of human B cells. The approach allows the unbiased
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