SOLID ChIP-Seq Kit with ChIP M

ChIP-Seq分析常见问题集锦

染色质免疫共沉淀测序（ChIP-Seq）是指对染色质免疫共沉淀（ChIP）获得的DNA片段进行大规模测序，并能把所研究蛋白的DNA结合位点精确定位到基因组上。Roche GS FLX Titanium 、Illumina Hiseq2000和AB SOLID 4 这3种测序技术均可以用于ChIP-seq，其中采用Illumina Hiseq2000进行ChIP-Seq已有较多文献报道。 ChIP-Seq技术高质量、高通量、低成本的数据产出，为表观遗传组学研究奠定了技术基础。研究

Quantitatively Profiling Genome-Wide Patterns of Histone Modifications in Arabidopsis thaliana Using ChIP-seq

including the lower amount of input DNA required, an expanded dynamic range and compatibility with sample multiplexing. Here we describe a detailed protocol for profiling histone modification in the Arabidopsis thaliana genome with ChIP-seq using the SOLiD™

Dynabeads® mRNA DIRECT Kit

and synthesis of first-strand cDNA (ref. 60), allowing the construction of solid-phase cDNA libraries and solid-phase RT-PCR. 实验试剂   Lysis/Binding Buffer Washing Buffer A Washing Buffer B 10 mM Tris-HCl (Elution Buffer