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- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Human nucleoporin 85kDa (NUP85), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
- 供应商:
OriGene
- 规格:
50 ug
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文献和实验Da are given in Table 1 . Table 1 R min for proteins of different mass Protein M (kDa
sterile water. Determine the concentration by UV spectroscopy (1 A260-unit is equivalent to 32 µg RNA) and continue with annealing (see below). It should be noted that the crude RNA products are more than 85% full-length, which makes gel-purification
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
with the Wnt preparation and cellular context. Key words: sFRP , Recombinant expression , Protein purification , Heparin chromatography , Chelating chromatography , Wnt 1. Introduction Secreted Frizzled-related proteins (sFRPs) contain a Frizzled(Fzd
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