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- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Human epiphycan (EPYC), with C-terminal His tag, secretory expressed in HEK293 cells, 50ug
- 供应商:
OriGene
- 规格:
50 ug
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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
by appropriate control experiments that the tagged protein is functional in vivo . Vectors for both N- and C-terminal fusion are available from IBA Tagnology (Germany), who has developed these tags and binding matrices. We recommend using the OneStrep-tag
Strategies to Optimize Protein Expression in E. coli
., Cantor, E.J., Liao, W., Xu, M.Q., and Benner, J. 1998. Utilizing the C‐terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26:5109‐5115
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides
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