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- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Mouse nei endonuclease VIII-like 1 (E. coli) (cDNA clone MGC:49102 IMAGE:5320651), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验) and tyrosine (which absorb less strongly than trp). vii) The pH of the crude cell lysate is kept at 5.3 to ensure that the protein will bind well to the SP column but not so low as to precipitate most of the E.coli proteins as well as CD2. Most E. coli
-based vectors for convenient and efficient use in E. coli . The use of these plasmids to carry out genetic engineering has been called recombinogenic engineering or recombineering (6). Recombineering exploits the phage derived protein pairs, either RecE
and infect E. coli at a much higher efficiency than plasmid transformation methods . Incomplete Digestion of Genomic DNA will allow identification of sequence overlaps Complete digestion with an endonuclease will result in a library
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