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- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Mouse RIKEN cDNA 0610010O12 gene (cDNA clone MGC:41247 IMAGE:1195860), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验alone (for blue/white selection of inserts within the B-galactosidase gene). Leave the solution to dry (>10 minutes) before plating 7. Leave plates ON at 37o C 8. Screen the colonies obtained to identify the desired clone
the generation of normalized, full?length?enriched cDNA libraries, and enabling efficient RNA analyses. Curr. Protoc. Mol. Biol. 90:5.12.1?5.12.27. © 2010 by John Wiley & Sons, Inc. Keywords: cDNA normalization; duplex?specific nuclease (DSN
cDNA Expression Cloning in Mammalian Cells
) Recombinant T7 (Fuerst et al., ) or SP6 (Usdin et al., ) vaccinia virus stock (available from B. Moss, NIH) Purified DNA from cDNA pools (see protocol 1 , steps
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