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- 详细信息
- 文献和实验
- 技术资料
- 浓度:
1mg/ml
- 应用范围:
科学研究
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Chicken,Dog,Cow,Rabbit,Sheep
- 标记物:
PE HRP Gold Cy5.5 Cy7 RBITC PE-CY5.5 PE-CY7 APC FITC Cy3 Cy3844
- 克隆性:
Polyclonal
- 保存条件:
-20℃
- 形态:
液态/冻干粉
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human/mouse/Rat
- 规格:
25 ug
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文献和实验CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
represent the migratory and inflammatory DC subtypes and not the DC subtypes found in the steady state. By contrast a different culture method was described where mouse bone marrow is cultured with flt3 ligand for 9 days. Here, we describe this method
The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen
immunofluorescent labeling and flow cytometric cell sorting. If the availability of mice is limiting, our protocol can cater for DC numbers boosted by the administration of fms-like tyrosine kinase 3 ligand (Flt3L), directly via subcutaneous injection
of many of these DC subpopulations from their precursors using bone marrow cultures supplemented with fms-like tyrosine kinase 3 ligand (Flt3L). The culture-generated DC can be aligned with the populations directly isolated from tissues. Combining the in vivo
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