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- 详细信息
- 文献和实验
- 技术资料
- 浓度:
1mg/ml
- 应用范围:
科学研究
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Chicken,Dog,Cow,Rabbit,Sheep
- 标记物:
PE HRP Gold Cy5.5 Cy7 RBITC PE-CY5.5 PE-CY7 APC FITC Cy3 Cy3844
- 克隆性:
Polyclonal
- 保存条件:
-20℃
- 形态:
液态/冻干粉
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human/mouse/Rat
- 规格:
25 ug
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文献和实验CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
represent the migratory and inflammatory DC subtypes and not the DC subtypes found in the steady state. By contrast a different culture method was described where mouse bone marrow is cultured with flt3 ligand for 9 days. Here, we describe this method
The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen
immunofluorescent labeling and flow cytometric cell sorting. If the availability of mice is limiting, our protocol can cater for DC numbers boosted by the administration of fms-like tyrosine kinase 3 ligand (Flt3L), directly via subcutaneous injection
FLT-3L,全称为FMS样酪氨酸激酶3配体,也被称为FLT3配体或Flt3L。其主要功能是通过与细胞表面的FLT3受体结合,激活下游的信号通路,从而调控造血干细胞的增殖、分化和存活。 FLT3L是一种分泌型糖蛋白,属于I型跨膜蛋白家族,其天然存在膜结合型和可溶性两种异构体——膜结合型可通过蛋白酶切释放,形成具有生物活性的可溶性FLT3L,二者均能与细胞表面的FLT3(CD135)受体特异性结合,激活下游信号通路。 Flt3-Ligand广泛表达于多种组织细胞,如骨髓基质细胞、成纤维
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