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文献和实验Characterization of Tachykinin Receptors
Cells and Tissues Materials Tachykinin peptide (>95% purity by HPLC; Bachem): e.g., Tyr−1 ‐substance P (Tyr−1 ‐SP), neurokinin A (NKA
Phenol Extraction of rRNA (Rat liver)
Homogenizer or blender6 Refrigerated preparative centrifuge NaCl 95% and 70% (v/v) ethanol Procedure Obtain a rat which has been fasted for 24 hours (to remove glycogen from the liver), decaptitate
Thymidine-Incorporation Assay for Rat-1a cells
Thymidine-Incorporation Assay for Rat-1a cells Overview This method of Peter Coward, Ph.D. in the Conklin Lab was used in Coward, et al (1998) Controlling signaling with a specifically designed Gi-coupled receptor Proc. Natl
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