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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
assays are the most appropriate for measuring the titer of the antisera you have generated. ELISA Procedure for Measuring Serum Antibody Titer In this type of ELISA (Enzyme-Linked Immunosorbant Assay), the antigen (peptide or protein) is bound
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
proteases, purify the antibody of interest if possible. Otherwise just freeze 100 uL aliquots and use as needed by thawing when needed. Don't refreeze.
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