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文献和实验Volume of Table MFT(0.5ml) MFT(1.5ml) Corex ORT 250ml DNA 105μl 210ul 315μl 2.1ml 9ml 60.0ml NH4Ac 35μl 70μl 105μl 0.7ml 3ml 12.6g EtOH 350μl 700μl 10μl 7.0ml 30ml 165.0ml Optional spin check: Min/rpm 15/14K 20/14K 10/14K 25
NH4Ac and EtOH precipitation of DNA
is resuspended in H2O:low salt in the resuspension buffer is important). Often multiple NH4Ac/EtOH precipitation are desired. If the DNA is particularly important or if it is at a very low concentration i.e. Sample Volume of Table MFT(0.5 ml)
NH4Ac and EtOH precipitation of DNA
NH4Ac and EtOH precipitation of DNA Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M, mix (spin at 4 C, transfer the supernatant to a new tube; optional spin for extra purification of the DNA), add
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