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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
(here we describe the preparation of cytosol from adult rat brains, Steps 1�8) and fluorescently labeled endosomes are needed (we label early endosomes by incubating cultured cells with externally added fluorescent cargo molecules by endocytotic uptake, Steps 9�24). After labeling
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