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文献和实验Monoclonal Antibody Production Protocol
of 1/5 dilutions. Do dilutions in blocking solution. Leave 1 hr at room temp or O.N at 4°C. SECONDARY ANTIBODY Wash unbound antibody 4 times with PBS + 0.1% Tween 20. Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa
Syntheses of DNA Duplexes That Contain a N4C‐Alkyl‐N4C Interstrand Cross‐Link
Webba da Silva, M., Noronha, A.M., Noll, D.M., Miller, P.S., Colvin, O.M., and Gamcsik, M.P. 2002. Solution structure of a DNA duplex containing mispair‐aligned N4C‐ethyl‐N4C interstrand cross‐linked cytosines. Biochemistry 41:15181‐15188
with the spheroplasts and pellet as above. Resuspend in 10 mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell
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