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文献和实验discrimination in Sprague‐Dawley rats. Panels (A ) and (B ) depict the acquisition of the nicotine (0.4 mg/kg, i.p.) DS in 3‐ to 4‐month‐old Sprague‐Dawley rats ( n = 18). Open circles represent saline‐pretreatment sessions, and closed circles nicotine
Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0. 2.0.5 mM ATP,2 mM MgCl2,100 mM boric acid,pH 8.3 at 4℃,10 ml. 3.N-(1-pyrene)iodoacetamide (Molecular probes P-29,m.w.385) 4.50Ti tubes,small vial
Fluorescence Mounting Medium (Antifade)
mixing. Weigh out 10 mg of p-phenylenediamine on the Mettler balance. PPD is toxic. Wear gloves and don’t inhale it. Add the PPD to the vial and stir untill it is all in solution (1-2 hrs.). The medium should appear almost colorless
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