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文献和实验, the principle of protein quantitation by ICAT has been combined with phosphoprotein enrichment (60). This was accomplished by the introduction of isotopic label into ethanedithiol, the reagent used to convert the alkene created by ß-elimination of phosphoserine
Gene (Ref) Accession # Position from ATG Gene Target (Comments) Cell Line Tested ß-actin (1) NM_001101 972-994 aatgaagatcaagatcattgc Human HeLa gamma-actin (1) NM_001614 8-30 aagagatcgccgcgctggtca Human HeLa AIB1 (estrogen receptor
Required Equipment Shallow trays, large enough to hold blot Glass plates Plastic wrap (e.g., Saran™ film), freezer bag, or sheet protector Autoradiography film and cassette Dark room Autoradiography film
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