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文献和实验. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months. I. Protease Inhibitor Cocktail The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution buffers**. Cocktail
before use. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months. I. Protease Inhibitor Cocktail The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution buffers
Development of techniques for primary culture of C. elegans embryonic neurons
number of individual cells were visible (60-100) strokes. The cells and embryos were then incubated in a cocktail of collagenase type IA, IV, and VII (Sigma; 0.1 mg/ml each in Ca/Mg-free medium) for 60 min at room temperature
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