pLV2-CMV-MCS-3×FLAG-Puro plasm

pLV2-CMV-MCS-3×FLAG-Puro plasmid

PVT112149

pLV2-CMV-MCS-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-MCS-3×FLAG-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-MCS-3×FLAG-Puro plasmid Description

pLV2-CMV-MCS-3×FLAG-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-MCS-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcACGC GTTGTACACC CGGGGGATCC gcggccgcGA CTACAAAGAC 2881 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagtct 2941 agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 3001 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 3061 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 3121 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 3181 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 3241 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 3301 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 3361 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 3421 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg 3481 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 3541 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 3601 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 3661 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 3721 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 3781 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 3841 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 3901 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 3961 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 4021 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc 4081 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 4141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 4201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 4261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 4321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 4381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 4441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 4501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 4561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 4621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga 4681 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag 4741 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag 4801 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 4861 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc 4921 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag 4981 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc 5041 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc 5101 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag 5161 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag 5221 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg 5281 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct 5341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 5401 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 5461 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 5521 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 5581 gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc 5641 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 5701 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 5761 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 5821 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 5881 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 5941 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 6001 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 6061 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 6121 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 6181 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 6241 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 6301 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 6361 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 6421 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 6481 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 6541 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 6601 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 6661 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 6721 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 6781 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 6841 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 6901 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 6961 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 7021 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 7081 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 7141 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 7201 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 7261 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 7321 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 7381 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 7441 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 7501 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 7561 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 7621 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 7681 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 7741 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 7801 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata 7861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 7921 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc 7981 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac 8041 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat 8101 gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta ctacaaactt 8161 agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.