pLV3-CMV-mCherry-HDAC9-3×FLAG-

pLV3-CMV-mCherry-HDAC9-3×FLAG-Puro plasmid

PVT112133

pLV3-CMV-mCherry-HDAC9-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-mCherry-HDAC9(human)-3×FLAG-Puro
Alias: NM_178425.4;ARCND4; HD7; HD7b; HD9; HDAC; HDAC7;HDAC7B; HDAC9B; HDAC9FL; HDRP; MITR
Gene lenght: 3207
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-mCherry-HDAC9-3×FLAG-Puro plasmid Description

pLV3-CMV-mCherry-HDAC9-3×FLAG-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-mCherry-HDAC9-3×FLAG-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA 2341 GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA 2401 GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA 2461 GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA 2521 GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG 2581 CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA 2641 CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC 2701 CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT 2761 GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG 2821 CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC 2881 CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT 2941 CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA 3001 CAAGggtgga ggcggatcag gaggcggtgg atctatgcac agtatgatca gctcagtgga 3061 tgtgaagtca gaagttcctg tgggcctgga gcccatctca cctttagacc taaggacaga 3121 cctcaggatg atgatgcccg tggtggaccc tgttgtccgt gagaagcaat tgcagcagga 3181 attacttctt atccagcagc agcaacaaat ccagaagcag cttctgatag cagagtttca 3241 gaaacagcat gagaacttga cacggcagca ccaggctcag cttcaggagc atatcaagtt 3301 gcaacaggaa cttctagcca taaaacagca acaagaactc ctagaaaagg agcagaaact 3361 ggagcagcag aggcaagaac aggaagtaga gaggcatcgc agagaacagc agcttcctcc 3421 tctcagaggc aaagatagag gacgagaaag ggcagtggca agtacagaag taaagcagaa 3481 gcttcaagag ttcctactga gtaaatcagc aacgaaagac actccaacta atggaaaaaa 3541 tcattccgtg agccgccatc ccaagctctg gtacacggct gcccaccaca catcattgga 3601 tcaaagctct ccacccctta gtggaacatc tccatcctac aagtacacat taccaggagc 3661 acaagatgca aaggatgatt tcccccttcg aaaaactgcc tctgagccca acttgaaggt 3721 gcggtccagg ttaaaacaga aagtggcaga gaggagaagc agccccttac tcaggcggaa 3781 ggatggaaat gttgtcactt cattcaagaa gcgaatgttt gaggtgacag aatcctcagt 3841 cagtagcagt tctccaggct ctggtcccag ttcaccaaac aatgggccaa ctggaagtgt 3901 tactgaaaat gagacttcgg ttttgccccc tacccctcat gccgagcaaa tggtttcaca 3961 gcaacgcatt ctaattcatg aagattccat gaacctgcta agtctttata cctctccttc 4021 tttgcccaac attaccttgg ggcttcccgc agtgccatcc cagctcaatg cttcgaattc 4081 actcaaagaa aagcagaagt gtgagacgca gacgcttagg caaggtgttc ctctgcctgg 4141 gcagtatgga ggcagcatcc cggcatcttc cagccaccct catgttactt tagagggaaa 4201 gccacccaac agcagccacc aggctctcct gcagcattta ttattgaaag aacaaatgcg 4261 acagcaaaag cttcttgtag ctggtggagt tcccttacat cctcagtctc ccttggcaac 4321 aaaagagaga atttcacctg gcattagagg tacccacaaa ttgccccgtc acagacccct 4381 gaaccgaacc cagtctgcac ctttgcctca gagcacgttg gctcagctgg tcattcaaca 4441 gcaacaccag caattcttgg agaagcagaa gcaataccag cagcagatcc acatgaacaa 4501 actgctttcg aaatctattg aacaactgaa gcaaccaggc agtcaccttg aggaagcaga 4561 ggaagagctt cagggggacc aggcgatgca ggaagacaga gcgccctcta gtggcaacag 4621 cactaggagc gacagcagtg cttgtgtgga tgacacactg ggacaagttg gggctgtgaa 4681 ggtcaaggag gaaccagtgg acagtgatga agatgctcag atccaggaaa tggaatctgg 4741 ggagcaggct gcttttatgc aacagccttt cctggaaccc acgcacacac gtgcgctctc 4801 tgtgcgccaa gctccgctgg ctgcggttgg catggatgga ttagagaaac accgtctcgt 4861 ctccaggact cactcttccc ctgctgcctc tgttttacct cacccagcaa tggaccgccc 4921 cctccagcct ggctctgcaa ctggaattgc ctatgacccc ttgatgctga aacaccagtg 4981 cgtttgtggc aattccacca cccaccctga gcatgctgga cgaatacaga gtatctggtc 5041 acgactgcaa gaaactgggc tgctaaataa atgtgagcga attcaaggtc gaaaagccag 5101 cctggaggaa atacagcttg ttcattctga acatcactca ctgttgtatg gcaccaaccc 5161 cctggacgga cagaagctgg accccaggat actcctaggt gatgactctc aaaagttttt 5221 ttcctcatta ccttgtggtg gacttggggt ggacagtgac accatttgga atgagctaca 5281 ctcgtccggt gctgcacgca tggctgttgg ctgtgtcatc gagctggctt ccaaagtggc 5341 ctcaggagag ctgaagaatg ggtttgctgt tgtgaggccc cctggccatc acgctgaaga 5401 atccacagcc atggggttct gcttttttaa ttcagttgca attaccgcca aatacttgag 5461 agaccaacta aatataagca agatattgat tgtagatctg gatgttcacc atggaaacgg 5521 tacccagcag gccttttatg ctgaccccag catcctgtac atttcactcc atcgctatga 5581 tgaagggaac tttttccctg gcagtggagc cccaaatgag gttggaacag gccttggaga 5641 agggtacaat ataaatattg cctggacagg tggccttgat cctcccatgg gagatgttga 5701 gtaccttgaa gcattcagga ccatcgtgaa gcctgtggcc aaagagtttg atccagacat 5761 ggtcttagta tctgctggat ttgatgcatt ggaaggccac acccctcctc taggagggta 5821 caaagtgacg gcaaaatgtt ttggtcattt gacgaagcaa ttgatgacat tggctgatgg 5881 acgtgtggtg ttggctctag aaggaggaca tgatctcaca gccatctgtg atgcatcaga 5941 agcctgtgta aatgcccttc taggaaatga gctggagcca cttgcagaag atattctcca 6001 ccaaagcccg aatatgaatg ctgttatttc tttacagaag atcattgaaa ttcaaagcaa 6061 gtattggaag tcagtaagga tggtggctgt gccaaggggc tgtgctctgg ctggtgctca 6121 gttgcaagag gagacagaga ccgtttctgc cctggcctcc ctaacagtgg atgtggaaca 6181 gccctttgct caggaagaca gcagaactgc tggtgagcct atggaagagg agccagcctt 6241 gGGATCCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 6301 TGACGATGAC AAGTAgAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA 6361 CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA 6421 GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG 6481 AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG 6541 GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC 6601 GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT 6661 GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG 6721 GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC 6781 TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT 6841 CCAAGCTGTG ACCGGCGCCT ACGCTAGATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC 6901 ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC 6961 GCCACGCGCC ACACCGTCGA CCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA 7021 CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC 7081 GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC 7141 GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC 7201 CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG 7261 CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC 7321 GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC 7381 GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG 7441 TGCATGACCC GCAAGCCCGG TGCCTGAgtc gacaatcaac ctctggatta caaaatttgt 7501 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 7561 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 7621 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 7681 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 7741 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 7801 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 7861 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc 7921 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 7981 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 8041 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 8101 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 8161 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 8221 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 8281 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 8341 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 8401 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 8461 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 8521 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 8581 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 8641 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 8701 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 8761 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 8821 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 8881 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 8941 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 9001 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 9061 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 9121 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 9181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 9241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 9301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 9361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 9421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 9481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 9541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 9601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 9661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 9721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 9781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 9841 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 9901 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 9961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 10021 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 10081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 10141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 10201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 10261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 10321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 10381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 10441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 10501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 10561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 10621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 10681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 10741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 10801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 10861 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 10921 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 10981 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 11041 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 11101 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 11161 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 11221 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 11281 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 11341 cagtcacgac gttgtaaaac gacggccagt gccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.