pCMV-Prkch-EGFP-Neo plasmid

pCMV-Prkch-EGFP-Neo plasmid

PVT112039

pCMV-Prkch-EGFP-Neo plasmid Information

Plasmid name: pCMV-Prkch(mouse)-EGFP-Neo
Alias: NM_008856.4;Pkch
Gene lenght: 2049
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Prkch-EGFP-Neo plasmid Description

pCMV-Prkch-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Prkch-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatgt cgtccggcac gatgaagttc aatggctatc 961 tgagggtccg catcggagag gctgtagggc tgcagcccac ccgctggtcc ctgcggcact 1021 cgctcttcaa aaagggccac cagctgctgg acccctacct gacggtgagc gtagaccagg 1081 tacgcgtggg ccagaccagc acaaagcaga agaccaacaa acccacctac aacgaggagt 1141 tctgcgccaa tgtcaccgac ggcggccacc tggagctagc cgtcttccac gagacgcccc 1201 tgggttatga ccactttgtg gccaactgca cgctgcagtt ccaggagctg ttgcgcacgg 1261 ctggtacctc ggacaccttc gagggctggg tggatctgga gcctgagggg aaagtgtttg 1321 tggtaataac cctaacaggg agtttcactg aagccactct ccagagagac cgcatcttca 1381 agcattttac caggaagcgc caaagggcta tgcgaagacg agtccatcaa gtgaacggac 1441 ataagttcat ggccacgtac ctgaggcagc ccacctactg ctctcattgc cgagagttca 1501 tctggggagt atttgggaaa cagggttatc aatgccaagt gtgcacctgc gtcgtccata 1561 aacgctgcca tcatctaatt gttacagcct gcacttgcca aaacaatatt aacaaagtgg 1621 atgccaagat tgcagaacag cggtttggca tcaacatccc acacaagttc aacgttcaca 1681 attacaaggt gcccacgttc tgtgaccact gtggctccct gctctggggg ataatgcgac 1741 aaggacttca gtgtaaaata tgtaagatga atgtacatat tcggtgtcag gcgaacgtgg 1801 ccccaaactg cggggtgaat gccgtggagc ttgccaagac cctggcaggg atgggtctcc 1861 aacccggaaa tatttctcca acctcgaaac tcatttccag gtcgacacta agacggcagg 1921 gaaaggaggg ctccaaagaa ggaaatggga tcggtgttaa ctcttccagc agattcggca 1981 tcgacaactt tgagttcatc cgggtgttgg ggaaggggag cttcgggaag gtgatgcttg 2041 ccaggataaa ggagacagga gaactgtacg ccgtgaaggt gctgaagaag gacgtgattc 2101 tgcaggatga tgatgtagag tgcaccatga ctgagaagag gatcctgtcc ttggcccgca 2161 accacccctt cctcacccag ctcttctgct gctttcagac tccagaccgt ctgttctttg 2221 tcatggagtt tgtgaacgga ggcgacctga tgttccacat ccaaaagtcc cgtcgtttcg 2281 atgaagcccg tgctcgtttc tacgccgcgg agatcatttc tgcactcatg ttcctacatg 2341 agaaaggtat catctataga gacttgaaac tggacaatgt gctattggac cacgaaggtc 2401 actgtaaact ggccgacttt ggaatgtgca aggaggggat ttgtaatggg gtcaccacag 2461 ccaccttctg cggtacaccc gactacattg ccccggagat ccttcaggag atgctgtatg 2521 gacctgcagt agactggtgg gccatgggtg tgttgcttta tgagatgctg tgcggacatg 2581 cgccctttga ggctgaaaat gaagatgacc tttttgaggc catactgaat gatgaagtcg 2641 tctaccccac ctggctccac gaagatgcca cagggatcct caagtctttc atgaccaaga 2701 accccaccat gcgcttgggc agcctgactc agggaggaga gcatgagatc ctgagacacc 2761 ctttctttaa ggaaatcgac tgggcccagt tgaaccatcg ccagttagag ccgcctttcc 2821 gacctagaat caaatcccga gaagatgtca gcaattttga cccagacttt ataaaagaag 2881 agcccgtctt aactccgatt gatgagggac atcttcctat gattaaccag gatgagttta 2941 gaaacttttc ctatgtgtca ccggaattgc aactgctcga gggcggtgga ggaagcggcg 3001 gaggtggatc tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 3061 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 3121 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 3181 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 3241 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 3301 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 3361 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 3421 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 3481 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 3541 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 3601 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 3661 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 3721 tgtacaagta gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta 3781 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 3841 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 3901 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 3961 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 4021 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 4081 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 4141 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 4201 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 4261 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 4321 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 4381 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 4441 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 4501 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 4561 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 4621 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 4681 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 4741 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 4801 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 4861 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 4921 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 4981 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 5041 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 5101 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 5161 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 5221 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 5281 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 5341 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 5401 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 5461 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 5521 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 5581 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 5641 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 5701 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 5761 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc 5821 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa 5881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 5941 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc 6001 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 6061 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 6121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 6181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 6241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 6481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 6541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 6601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 6661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 6721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 6781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 6841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 6901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt 6961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 7441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 7501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 7561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 7621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 7681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 7741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 7801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 7861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 7921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 7981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8161 cctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.