pCMV-Sema6a-3×FLAG-Neo plasmid

pCMV-Sema6a-3×FLAG-Neo plasmid

PVT112023

pCMV-Sema6a-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Sema6a(mouse)-3×FLAG-Neo
Alias: NM_018744.2;A730020P05Rik; Sema6A-1; Semaq; VIa
Gene lenght: 3093
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Sema6a-3×FLAG-Neo plasmid Description

pCMV-Sema6a-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Sema6a-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgcg gccagcagcc ttactgctgt 781 gtctcacact gctacactgc gctggggctg gtttcccaga agattccgag ccaatcagta 841 tttcgcatgg caactataca aaacagtatc cggtgtttgt gggccacaag ccaggacgga 901 acaccacgca gaggcacagg ctggacatcc agatgatcat gatcatgaac agaaccctct 961 acgttgctgc tcgagaccat atttatactg ttgatataga cacatcccac acagaagaaa 1021 tttactgtag caaaaaactg acatggaaat ctagacaggc tgacgtagac acatgcagga 1081 tgaaggggaa acataaggat gaatgtcaca acttcattaa agttcttctc aagaagaatg 1141 atgatacgct gtttgtctgt ggaaccaatg ccttcaaccc ttcctgcaga aactacaggg 1201 tcgatacctt ggaaactttt ggggatgaat ttagcggaat ggccagatgc ccttatgatg 1261 ccaaacatgc caacatcgct ctgtttgcag atggaaaact ctactcggct acagtgactg 1321 actttctggc cattgatgca gtcatttaca ggagcctcgg agacagccct accctcagga 1381 ctgtcaagca tgattcaaag tggttgaaag agccgtactt tgtccaagcc gtggattatg 1441 gggactatat ctacttcttc ttcagagaaa ttgcagtaga atacaacact atggggaagg 1501 ttgttttccc tagggtggct caggtctgta agaatgacat gggagggtct cagagagtcc 1561 tggagaagca gtggacatct ttcctgaagg ctcgcctgaa ctgctcggtg cctggagact 1621 ctcattttta tttcaatata ctccaggcag ttacagatgt gattcgcatt aatggccgtg 1681 atgttgtctt ggcaaccttt tccacacctt ataacagcat cccaggttct gcagtctgtg 1741 cctatgacat gcttgacatt gctaatgttt tcactgggag gttcaaggaa cagaaatcac 1801 ctgactctac ctggacaccc gttccagacg aacgagtccc taagcccagg ccaggctgtt 1861 gtgctggatc atcctcttta gaaaaatatg caacctccaa tgagtttccc gatgataccc 1921 tgaacttcat taagacgcat ccactcatgg acgaggcagt gccttccatc atcaacagac 1981 cttggttcct gagaacaatg gtcagatacc gcctgaccaa aattgcagta gacaacgctg 2041 ccgggccata tcagaatcac actgtggttt tcctgggatc agaaaaggga atcatcctga 2101 agttcttggc caggatagga agcagtggtt tcctaaatgg cagccttttc ctggaggaga 2161 tgaatgttta caacccagaa aagtgcagct atgatggtgt agaagacaaa aggatcatgg 2221 gcatgcagct cgacagagcg agtggctcac tctatgttgc attctctact tgtgtgatca 2281 aggtgcctct tggccgctgt gagcgacatg ggaagtgtaa aaaaacctgc atcgcctcca 2341 gagacccgta ttgtgggtgg gtaagggaaa gtggttcctg tgcccatctg tcacccctta 2401 gcagactgac atttgagcag gacattgagc gtggcaatac ggacggccta ggagactgtc 2461 acaattcctt cgtggcactg aatgggcacg ccagttccct ctatcccagc accactacgt 2521 cagattcggc atcccgagac gggtatgagt ctaggggagg catgctggac tggaacgacc 2581 tgctcgaggc acctggcagc acagaccctt tgggggcagt gtcctctcat aaccaccagg 2641 acaagaaggg agtgattcgg gaaagttacc tcaaaagcaa cgaccagctt gttcctgtca 2701 ccctcctggc cattgcagtc attctggctt ttgtcatggg ggccgtcttc tcgggcatca 2761 tcgtgtattg tgtgtgcgat caccggcgca aagacgtggc agtagtgcag cgcaaggaga 2821 aagagctcac tcactcgcgt cggggatcta tgagcagtgt caccaagctc agtggcctct 2881 ttggggacac ccagtccaag gacccaaagc ctgaggccat cctcacacca ctcatgcaca 2941 acggcaagct ggccacgcct agcaacaccg ccaagatgct catcaaggct gaccagcatc 3001 acctagacct caccgccctg cccaccccag agtccacccc gacactgcag cagaaacgga 3061 aacccaaccg cggcagtcgc gagtgggaga ggaaccagaa catcatcaat gcctgcacca 3121 aggacatgcc tcccatgggt tcccctgtga ttcccacgga cctgcccctc cgggcctccc 3181 caagccacat ccccagcgtg gtggtcctgc ccatcacgca gcagggctac cagcacgagt 3241 acgtagatca gcccaaaatg agcgaggtgg tggctcagat ggcactggag gaccaggctg 3301 ccaccctgga gtataagacc atcaaagagc acctgagtag caagagtccc aaccatgggg 3361 tgaaccttgt ggagaacctg gacagcctgc cccctaaagt tccacagcgc gaggcctccc 3421 taggtccccc gggaacctca ctgtcacaga ccggcctgag caagaggctg gagatgcaac 3481 actcctcctc ctatgggctc gaatataaga ggagctaccc cacgaactcg ctcacaagaa 3541 gccaccagac caccactctc aaaagaaaca atactaactc ctccaattcc tcccacctct 3601 ccaggaacca gagctttggc cggggagaca acccaccccc cgccccgcag cgggtggact 3661 ctatccaggt gcacagctcc cagccctctg gccaggccgt gactgtttcg aggcagccca 3721 gcctcaatgc ctacaactca ctgacgaggt cggggctgaa gcgcaccccc tcgctaaagc 3781 cagatgtacc ccccaaacct tcctttgctc ccctttccac atccatgaag cccaatgatg 3841 catgtacaGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT 3901 ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA 3961 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 4021 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 4081 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 4141 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 4201 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 4261 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 4321 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG 4381 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 4441 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 4501 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA 4561 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA 4621 ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC 4681 TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC 4741 ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG 4801 CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 4861 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 4921 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 4981 TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 5041 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 5101 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 5161 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG 5221 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 5281 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5341 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 5401 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 5461 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 5521 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 5581 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 5641 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 5701 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 5761 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 5821 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 5881 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 5941 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 6001 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 6061 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 6121 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 6181 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 6241 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 6301 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 6361 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 6421 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 6481 GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA 6541 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 6601 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT 6661 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 6721 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 6781 TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 6841 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 6901 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 6961 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 7021 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 7081 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 7141 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 7201 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 7261 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 7321 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 7381 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA 7441 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 7501 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 7561 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 7621 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 7681 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 7741 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 7801 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 7861 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 7921 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 7981 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 8041 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 8101 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 8161 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 8221 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8281 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8341 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8401 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8461 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8521 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8581 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8641 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 8701 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 8761 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 8821 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 8881 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 8941 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 9001 CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 9061 TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT 9121 ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.