pC3-Fluc plasmid

pC3-Fluc plasmid

PVT111979

pC3-Fluc plasmid Information

Plasmid name: pC3(human)-Fluc
Alias: NG_009557.1
Gene lenght: 3021
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: C3
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: Fluc（special construction）
Copy number: High
Induction:

pC3-Fluc plasmid Description

pC3-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pC3-Fluc plasmid Sequence

1 ggtacctcag taaagacggg gtttcaccat gttggccagg ctggtctcca actcctggcc 61 tcaagtgatc tgcctgcctc ggcctcccaa agtgctggga ttacagacgt gagccactgc 121 gcctggcaag gggatgcagt ttcaaaagct gaaccccaat tctggagagc aagcaggtat 181 tttcattctc tctcctcctc ctcctcctct tccaaagagt gtgtcgcaat cagtgcagac 241 agacgccagg tttgttctca tgctccacgc ctccccctac ccctggcacg gaaaagaatg 301 tggtttacag gaaatcagag aaaactcccc attaacccct tcagtggggt ttcagaaacc 361 gcctctccag ggataagggg gccccaccca cagacccttc tcctgccctc accatccacc 421 tcgtatgcct gggcagcaat gctgcagaac gtcagaggaa tgccagttaa aatgacaccg 481 gctgccgggg tgtggtggct cacttctata atcccagcac tctgggaggc cgaggtgggc 541 ggatcacctg aggtcaggag tttgagacca gcctggccaa catggcgaaa ccctttctct 601 actaaaaata caaaaaataa aaaataaaag aaaaaaaaaa ttagccaggt gtagtggcgc 661 atgcctgtga tcccagctct ttgggaggct gaggcaggag aatcacttga acccaggagg 721 cagaggttgc agtgagctga gatggcgcca ctgcactcca ccctgggtga cagcacaaga 781 ctccatttaa aaaaacaaaa caaaacaaaa aaaatgacac cagggtacca gttttcaccc 841 ataaggctgg caaaaatctt caagttcatc aacatgccct tgtgatgagg ctgtggaaga 901 aactgacaat tcatttcatg cagggctcat aagtgtgtaa atcaatacaa cttctgtgca 961 ggggaatttg gcaatatcta gcaagattac cagtgcattc agagattgac ccaacatatt 1021 tcctttcatt gcaacgacaa ctctatgaag caggtggtaa gggtttcctt ttccatgaac 1081 aaactgaggc tcagggcggt aatcagtagc ttacccaaag atcacagcta gtttcagagc 1141 tagaaaataa cgcaggttca agcttattca ctgcagagag cctggtgtga agccacagat 1201 gtcagtctct ccatcaagaa gaggctggtg gctggacaca gcggctcacg cctgtaattc 1261 caacactttg ggaggccaag gtaggtgggt cacttgaagt caggagttca agaccagcct 1321 ggccaacatg gtgaaacccc ttgtccacta aaaatacaaa aattgccaga cgtggtggtg 1381 ctcacctata atttcagcca ttccggaggc tgaggcagga gaattgtttg aacccaggag 1441 gtggagggtg cagtgagctg agatagcgcc actgccctcc agcctgggtg acagggcaag 1501 actctaaaaa aaaaacactc aaacaaacaa aatatccccc aaaaagtagg aggctggtta 1561 ctttctcaca atataacaag aggcctgtaa cctgtaagaa tgaggcagtt ctttgctcac 1621 tgaggtgaaa tagcctctga ggtatattgt tcatgaaaaa acgaaacaaa acgaaaccca 1681 agatttaact gaagagacca ggaagaatag tatgtgctat gtgctgtcca cagggcacag 1741 tagttcacac cagcactttg tgaggctgct gcgggaggat cacttgagcc caggagttca 1801 agactggact gggcaacatc gtgggacccc catctccaca aaaataaaaa aattatccgg 1861 gcatggtggc ggccacccgt agtcccggct acttgggtgg ttgagccagg atgatcactt 1921 gaccccagga ggttgaggct gcagtgagct gtgattgcac cactgcaatt tagcctgagt 1981 gacagaatga aaaaaaaatt tttttaaagg aaaacacaaa aagaatatgc tgtcaacagg 2041 gatgggagga agaccacctt tactgctata cacatttgta ccttttagat gttgatcaat 2101 atgaatatat tatacacaca gacacacaca cagacacaca cacacacaca aacaatacaa 2161 tttaatatcc taagaggata ttgacattag acaggtacaa aagctctaga aatgaggact 2221 ttcctcagtg atgacttttt tcaccaccaa agtcactcag gcatcctgac aagggtaagt 2281 gaggggagcc tccttggaaa ataaactcac ttggatagtg aactcctgca catacctcaa 2341 agcccatctg aaatgtcccc tcctacagga agttttccct gaccctccaa gaagcagagt 2401 tctatttcac tggggaaaac atttcttctt cttctttttt ttccctgccc tgcacatgag 2461 ctagaaaaca tttcatgaaa ctgggagttt ctgtgctggg ctctgtccct cccccattct 2521 acttcccctc cctcagcatg gaagcctctg gaagtggggc tctgactccc agcctacaga 2581 gagattccta ggaagtgttc gactgataaa cgcatggcca aaagtgaact ggggatgagg 2641 tccaagacat ctgcggtggg gggttctcca gaccttagtg ttcttccact acaaagtggg 2701 tccaacagag aaaggtctgt gttcaccagg tggccctgac cctgggagag tccagggcag 2761 ggtgcagctg cattcatgct gctggggaac atgccctcag gttactcacc ccatggacat 2821 gttggcccca gggactgaaa agcttaggaa atggtattga gaaatctggg gcagccccaa 2881 aaggggagag gccatgggga gaaggggggg ctgagtgggg gaaaggcagg agccagataa 2941 aaagccagct ccagcaggcg ctgctcactc ctccccatcc tctccctctg tccctctgtc 3001 cctctgaccc tgcactgtcc cagcaccaag cttggcattc cggtactgtt ggtaaagcca 3061 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg 3121 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa 3181 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt 3241 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg 3301 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag 3361 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca 3421 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc 3481 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg 3541 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg 3601 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg 3661 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct 3721 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg 3781 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat 3841 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt 3901 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc 3961 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct 4021 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat 4081 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg 4141 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga 4201 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg 4261 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt 4321 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt 4381 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc 4441 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac 4501 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg 4561 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg 4621 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca 4681 taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc ggccggccgc 4741 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 4801 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 4861 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 4921 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg 4981 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg 5041 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag 5101 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 5161 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 5221 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 5281 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 5341 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 5401 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 5461 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 5521 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 5581 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 5641 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 5701 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 5761 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 5821 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 5881 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 5941 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 6001 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 6061 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 6121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 6181 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 6241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 6301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 6361 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 6421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 6481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 6541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 6601 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 6661 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 6721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 6781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 6841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 6901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 6961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 7021 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 7081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 7141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 7201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 7261 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 7321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 7381 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 7441 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 7501 ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 7561 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact 7621 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt 7681 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca 7741 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.