pLV3-CMV-KCNA1-3×FLAG-CopGFP-P

pLV3-CMV-KCNA1-3×FLAG-CopGFP-Puro plasmid

PVT111743

pLV3-CMV-KCNA1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KCNA1(human)-3×FLAG-CopGFP-Puro
Alias: NM_000217.3;AEMK; EA1; HBK1; HUK1; KV1.1; MBK1; MK1;RBK1
Gene lenght: 1485
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KCNA1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-KCNA1-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KCNA1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga cggtgatgtc tggggagaac 2341 gtggacgagg cttcggccgc cccgggccac ccccaggatg gcagctaccc ccggcaggcc 2401 gaccacgacg accacgagtg ctgcgagcgc gtggtgatca acatctccgg gctgcgcttc 2461 gagacgcagc tcaagaccct ggcgcagttc cccaacacgc tgctgggcaa ccctaagaaa 2521 cgcatgcgct acttcgaccc cctgaggaac gagtacttct tcgaccgcaa ccggcccagc 2581 ttcgacgcca tcctctacta ctaccagtcc ggcggccgcc tgcggaggcc ggtcaacgtg 2641 cccctggaca tgttctccga ggagatcaag ttttacgagt tgggcgagga ggccatggag 2701 aagttccggg aggacgaggg cttcatcaag gaggaggagc gccctctgcc cgagaaggag 2761 taccagcgcc aggtgtggct gctcttcgag taccccgaga gctcggggcc cgccagggtc 2821 atcgccatcg tctccgtcat ggtcatcctc atctccatcg tcatcttttg cctggagacg 2881 ctccccgagc tgaaggatga caaggacttc acgggcaccg tccaccgcat cgacaacacc 2941 acggtcatct acaattccaa catcttcaca gaccccttct tcatcgtgga aacgctgtgt 3001 atcatctggt tctccttcga gctggtggtg cgcttcttcg cctgccccag caagacggac 3061 ttcttcaaaa acatcatgaa cttcatagac attgtggcca tcattcctta tttcatcacg 3121 ctgggcaccg agatagctga gcaggaagga aaccagaagg gcgagcaggc cacctccctg 3181 gccatcctca gggtcatccg cttggtaagg gtttttagaa tcttcaagct ctcccgccac 3241 tctaagggcc tccagatcct gggccagacc ctcaaagcta gtatgagaga gctagggctg 3301 ctcatctttt tcctcttcat cggggtcatc ctgttttcta gtgcagtgta ctttgccgag 3361 gcggaagaag ctgagtcgca cttctccagt atccccgatg ctttctggtg ggcggtggtg 3421 tccatgacca ctgtaggata cggtgacatg taccctgtga caattggagg caagatcgtg 3481 ggctccttgt gtgccatcgc tggtgtgcta acaattgccc tgcccgtacc tgtcattgtg 3541 tccaatttca actatttcta ccaccgagaa actgaggggg aagagcaggc tcagttgctc 3601 cacgtcagtt cccctaactt agcctctgac agtgacctca gtcgccgcag ttcctctact 3661 atgagcaagt ctgagtacat ggagatcgaa gaggatatga ataatagcat agcccattat 3721 agacaggtca atatcagaac tgccaattgc accactgcta accaaaactg cgttaataag 3781 agcaagctac tgaccgatgt tggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3841 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 3901 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3961 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 4021 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 4081 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4141 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4201 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4261 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4321 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4381 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4441 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4501 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 4561 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4621 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4681 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4741 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4801 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4861 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 4921 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 4981 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 5041 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 5101 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 5161 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 5221 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 5281 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5341 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5401 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5461 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5521 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5581 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5641 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5701 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5761 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5821 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 5881 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5941 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 6001 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 6061 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6121 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6181 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6241 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 6301 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6361 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 6421 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6481 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6541 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6721 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6781 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6961 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 7021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 7081 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 7141 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7981 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 8041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 8101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 8161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 8221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 8281 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 8341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 9001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 9061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 9121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 9181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 9241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 9301 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 9361 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 9421 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9481 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9541 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9601 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9661 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9721 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.