pCMV-NUPR1-Neo plasmid

pCMV-NUPR1-Neo plasmid

PVT111465

pCMV-NUPR1-Neo plasmid Information

Plasmid name: pCMV-NUPR1(human)-Neo
Alias: NM_001042483.2;COM1; P8
Gene lenght: 300
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-NUPR1-Neo plasmid Description

pCMV-NUPR1-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-NUPR1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggccacctt 961 cccaccagca accagcgccc cccagcagcc cccaggcccg gaggacgagg actccagcct 1021 ggatgaatct gacctctata gcctggccca ttcctacctc gggcctctca tcatgcctat 1081 gcccacttca cctctgactc ctgccttggt tacaggaggt ggaggccgga aaggtcgcac 1141 caagagagaa gctgctgcca acaccaaccg ccccagccct ggcgggcacg agaggaaact 1201 ggtgaccaag ctgcagaatt cagagaggaa gaagcgaggg gcacggcgct gactcgagca 1261 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct 1321 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1381 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1441 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1501 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 1561 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 1621 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 1681 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 1741 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 1801 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 1861 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 1921 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt 1981 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 2041 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc 2101 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 2161 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 2221 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 2281 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 2341 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 2401 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 2461 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 2521 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 2581 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 2641 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 2701 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 2761 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 2821 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 2881 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 2941 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 3001 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 3061 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 3121 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 3181 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 3241 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 3301 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 3361 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 3421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 3481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 3541 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 3601 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 3661 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 3721 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 3781 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 3841 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 3901 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 3961 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 4021 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 4081 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 4141 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta 4201 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 4261 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 4321 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 4381 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 4441 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4501 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4561 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 4621 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 4681 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 4741 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4801 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4861 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4921 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4981 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 5041 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 5101 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 5161 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 5221 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 5281 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 5341 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 5401 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 5461 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5521 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5581 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 5701 atttccccga aaagtgccac ctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.