pCMV-3×FLAG-SUMO1-Neo plasmid

pCMV-3×FLAG-SUMO1-Neo plasmid

PVT99884

pCMV-3×FLAG-SUMO1-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-SUMO1(human)-Neo
Alias: NM_003352.8;DAP1; GMP1; OFC10; PIC1; SENP2; SMT3;SMT3C; SMT3H3; UBL1
Gene lenght: 303bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-SUMO1-Neo plasmid Description

pCMV-3×FLAG-SUMO1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-SUMO1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atgtctgacc aggaggcaaa accttcaact 1021 gaggacttgg gggataagaa ggaaggtgaa tatattaaac tcaaagtcat tggacaggat 1081 agcagtgaga ttcacttcaa agtgaaaatg acaacacatc tcaagaaact caaagaatca 1141 tactgtcaaa gacagggtgt tccaatgaat tcactcaggt ttctctttga gggtcagaga 1201 attgctgata atcatactcc aaaagaactg ggaatggagg aagaagatgt gattgaagtt 1261 tatcaggaac aaacgggggg tcattcaaca gtttaggaat tctgcagata tccagcacag 1321 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct 1381 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 1441 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 1501 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 1561 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 1621 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 1681 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 1741 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 1801 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 1861 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 1921 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 1981 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 2041 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 2101 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2161 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2221 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2281 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2341 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2401 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 2461 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 2521 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 2581 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 2641 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 2701 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 2761 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 2821 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 2881 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 2941 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 3001 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 3061 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 3121 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 3181 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 3241 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 3301 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 3361 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 3421 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 3481 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 3541 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 3601 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 3661 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 3721 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 3781 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 3841 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 3901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 3961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4201 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4441 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 4501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtGG 4561 Tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 4681 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 4741 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 4801 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 4861 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 4921 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 4981 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 5041 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 5101 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 5161 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 5221 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 5281 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 5341 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 5401 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 5461 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 5521 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 5581 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 5641 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 5701 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 5761 agtgccacct gacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.