pCMV-MTCH2-V5-Neo plasmid

pCMV-MTCH2-V5-Neo plasmid

PVT111453

pCMV-MTCH2-V5-Neo plasmid Information

Plasmid name: pCMV-MTCH2(human)-V5-Neo
Alias: NM_014342.4;HSPC032; MIMP; SLC25A50
Gene lenght: 909
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-MTCH2-V5-Neo plasmid Description

pCMV-MTCH2-V5-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-MTCH2-V5-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gccaccatgg cggacgcggc cagtcaggtg ctcctgggct ccggtctcac catcctgtcc 961 cagccgctca tgtacgtgaa agtgctcatc caggtgggat atgagcctct tcctccaaca 1021 ataggacgaa atatttttgg gcggcaagtg tgtcagcttc ctggtctctt tagttatgct 1081 cagcacattg ccagtatcga tgggaggcgc gggttgttca caggcttaac tccaagactg 1141 tgttcgggag tccttggaac tgtggtccat ggtaaagttt tacagcatta ccaggagagt 1201 gacaagggtg aggagttagg acctggaaat gtacagaaag aagtctcatc ttcctttgac 1261 cacgttatca aggagacaac tcgagagatg atcgctcgtt ctgctgctac cctcatcaca 1321 catcccttcc atgtgatcac tctgagatct atggtacagt tcattggcag agaatccaag 1381 tactgtggac tttgtgattc cataataacc atctatcggg aagagggcat tctaggattt 1441 ttcgcgggtc ttgttcctcg ccttctaggt gacatccttt ctttgtggct gtgtaactca 1501 ctggcctacc tcgtcaatac ctatgcactg gacagtgggg tttctaccat gaatgaaatg 1561 aagagttatt ctcaagctgt cacaggattt tttgcgagta tgttgaccta tccctttgtg 1621 cttgtctcca atcttatggc tgtcaacaac tgtggtcttg ctggtggatg ccctccttac 1681 tccccaatat atacgtcttg gatagactgt tggtgcatgc tacaaaaaga ggggaatatg 1741 agccgaggaa atagcttatt tttccggaag gtcccctttg ggaagactta ttgttgtgac 1801 ctgaaaatgt taattaagct tatgggtaag cctatcccta accctctcct cggtctcgat 1861 tctacatagg atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg 1921 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag 1981 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 2041 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 2101 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 2161 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 2221 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 2281 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 2341 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 2401 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 2461 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 2521 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 2581 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 2641 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 2701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2761 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2821 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 2881 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 2941 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 3001 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga 3061 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 3121 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 3181 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 3241 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 3301 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 3361 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 3421 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 3481 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 3541 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 3601 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 3661 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 3721 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 3781 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 3841 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 3901 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3961 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 4021 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 4081 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4141 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 4201 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 4261 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 4321 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 4381 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 4441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 4501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 4561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 4621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 4681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 4741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 4801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 4861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 4921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 4981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 5041 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 5101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg 5161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 5221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 5281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 5341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 5401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 5461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 5521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 5581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 5641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 5701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 5761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 5821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 5881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 5941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 6001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 6061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 6121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 6181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 6241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 6301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 6361 ctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.