pLV3-CMV-TMEM43-3×HA-CopGFP-Pu

pLV3-CMV-TMEM43-3×HA-CopGFP-Puro plasmid

PVT107158

pLV3-CMV-TMEM43-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TMEM43(human)-3×HA-CopGFP-Puro
Alias: NM_024334.3;ARVC5; ARVD5; AUNA2; AUNA3; EDMD7; LUMA
Gene lenght: 1200
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TMEM43-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-TMEM43-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-TMEM43-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccgcgaatta ttccagtacc 2341 agtacccgga gagaacatgt caaagttaaa accagctccc agccaggctt cctggaacgg 2401 ctgagcgaga cctcgggtgg gatgtttgtg gggctcatgg ccttcctgct ctccttctac 2461 ctaattttca ccaatgaggg ccgcgcattg aagacggcaa cctcattggc tgaggggctc 2521 tcgcttgtgg tgtctcccga cagcatccac agtgtggctc cggagaatga aggaaggctg 2581 gtgcacatca ttggcgcctt acggacatcc aagcttttgt ctgatccaaa ctatggggtc 2641 catcttccgg ctgtgaaact gcggaggcac gtggagatgt accaatgggt agaaactgag 2701 gagtccaggg agtacaccga ggatgggcag gtgaagaagg agacgaggta ttcctacaac 2761 actgaatgga ggtcagaaat catcaacagc aaaaacttcg accgagagat tggccacaaa 2821 aaccccagtg ccatggcagt ggagtcattc atggcaacag ccccctttgt ccaaattggc 2881 aggtttttcc tctcgtcagg cctcatcgac aaagtcgaca acttcaagtc cctgagccta 2941 tccaagctgg aggaccctca tgtggacatc attcgccgtg gagacttttt ctaccacagc 3001 gaaaatccca agtatccaga ggtgggagac ttgcgtgtct ccttttccta tgctggactg 3061 agcggcgatg accctgacct gggcccagct cacgtggtca ctgtgattgc ccggcagcgg 3121 ggtgaccagc tagtcccatt ctccaccaag tctggggata ccttactgct cctgcaccac 3181 ggggacttct cagcagagga ggtgtttcat agagaactaa ggagcaactc catgaagacc 3241 tggggcctgc gggcagctgg ctggatggcc atgttcatgg gcctcaacct tatgacacgg 3301 atcctctaca ccttggtgga ctggtttcct gttttccgag acctggtcaa cattggcctg 3361 aaagcctttg ccttctgtgt ggccacctcg ctgaccctgc tgaccgtggc ggctggctgg 3421 ctcttctacc gacccctgtg ggccctcctc attgccggcc tggcccttgt gcccatcctt 3481 gttgctcgga cacgggtgcc agccaaaaag ttggagCTCG AGTACCCATA CGACGTACCT 3541 GATTATGCAA CTTATCCTTA TGATGTACCT GATTATGCTA GCTATCCTTA TGATGTCCCA 3601 GACTACGCAT AGggatccCT GCGATATCCA GCgcggccgc Gaaggatctg cgatcgctcc 3661 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3721 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3781 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3841 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3901 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3961 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4021 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4081 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4141 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4201 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4261 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4321 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4381 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4441 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4501 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4561 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4621 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4681 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4741 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4801 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4861 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4921 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4981 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5041 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5101 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5161 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5221 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5281 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5341 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5401 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5461 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5521 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5581 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5641 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5701 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5761 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5821 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5881 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5941 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6001 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 6061 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6121 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc 6181 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat 6241 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa 6301 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 6361 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 6421 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 6481 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 6541 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 6601 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 6661 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 6721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 6781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 6841 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt 6901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 6961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 7021 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 7081 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 7141 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 7201 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 7261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 7321 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 7381 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 7441 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 7501 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 7561 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 7621 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 7681 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 7741 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 7801 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 7861 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7921 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7981 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 8041 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 8101 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 8161 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 8221 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 8281 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 8341 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 8401 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 8461 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 8521 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 8581 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8641 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8701 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8761 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 8821 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8881 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8941 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 9001 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 9061 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 9121 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 9181 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 9241 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 9301 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 9361 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 9421 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 9481 cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.