pLV3-CMV-Ly75-3×HA-T2A-mCherry

pLV3-CMV-Ly75-3×HA-T2A-mCherry-Fluc-Puro plasmid

PVT111420

pLV3-CMV-Ly75-3×HA-T2A-mCherry-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-Ly75(mouse)-3×HA-T2A-mCherry-Fluc-Puro
Alias: NM_013825.4;CD205; DEC-205; DEC205
Gene lenght: 5169
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc,Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ly75-3×HA-T2A-mCherry-Fluc-Puro plasmid Description

pLV3-CMV-Ly75-3×HA-T2A-mCherry-Fluc-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Ly75-3×HA-T2A-mCherry-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagccacca tgcggacggg ccgggtgacc ccgggcctgg cggcggggct actcctgctg 2341 ttgctgcggt ccttcgggct tgtggagcct tctgagagct caggtaatga tccattcacc 2401 atcgtccatg aaaacactgg caagtgcatc cagccgctgt ctgactgggt agtggcccag 2461 gactgtagcg gaactaacaa catgttgtgg aagtgggtgt cccagcaccg cctctttcac 2521 ctggaatccc agaagtgcct cggcctcgat attaccaaag ccacggacaa cctgcgaatg 2581 ttcagctgtg actccaccgt catgctgtgg tggaaatgtg agcaccattc gctgtacacc 2641 gctgcccagt acaggctagc tctgaaagat ggatatgccg tagccaatac gaatacatct 2701 gatgtctgga agaagggagg ctccgaggaa aacctttgtg cccagcctta tcatgagata 2761 tacaccagag atgggaattc ctacgggaga ccttgtgaat tccctttctt gattggtgag 2821 acatggtacc atgactgcat tcatgatgaa gatcatagtg ggccatggtg tgccactacc 2881 ctaagttatg aatatgatca aaagtggggc atctgcctac taccagaaag tggctgtgaa 2941 ggtaactggg aaaagaatga gcagattgga agttgctacc aatttaataa tcaggaaatt 3001 ctgtcttgga aagaagctta tgtttcctgt cagaaccaag gagctgactt actgagcatc 3061 cacagtgctg ccgaattagc ctacattacg ggaaaagagg acattgctag acttgtttgg 3121 cttggactga atcagctcta ttctgcgaga ggttgggaat ggtcagactt caggccactc 3181 aaatttctta actgggatcc aggcacgccc gttgcacctg tgattggtgg gtcaagctgt 3241 gccagaatgg acacagagtc cgggctgtgg caaagtgttt cctgtgaatc tcagcagcct 3301 tacgtctgca agaagccact gaacaacacg ctggagctcc cagatgtttg gacttacaca 3361 gatacccact gccatgtggg ctggctgcca aataatgggt tttgctatct gctggcgaat 3421 gaaagtagtt cctgggatgc agcacatttg aaatgcaaag ccttcggtgc agacctcatc 3481 agcatgcact ccttagcaga tgtggaggtg gttgtcacga aactccataa tggggatgtc 3541 aaaaaagaaa tatggacagg ccttaaaaac acaaacagcc ctgctttgtt ccagtggtcg 3601 gacggaacgg aagttactct aacgtactgg aatgagaatg agccgagtgt tcccttcaac 3661 aagactccca actgtgtttc ctatttagga aagttaggtc agtggaaagt ccagtcctgt 3721 gagaagaaac tcagatatgt atgcaagaaa aagggagaaa taactaagga tgcagagtcg 3781 gataagctgt gtccgccaga cgagggctgg aagagacatg gagaaacctg ttacaagatt 3841 tatgagaaag aggccccttt cggaacgaac tgcaacctga ccatcactag caggttcgag 3901 caggaattct tgaattatat gatgaagaac tatgataagt cccttcggaa gtacttctgg 3961 actggcctga gagaccctga ctctcgagga gaatacagtt gggccgttgc tcagggagta 4021 aagcaggctg tgaccttttc caactggaat tttcttgaac cggcgtctcc aggcgggtgc 4081 gtggctatgt ctactggaaa gactcttggc aagtgggaag tgaagaactg cagaagcttc 4141 cgtgctcttt caatatgcaa gaaagtgagc gaaccccagg agcctgaaga agcagccccc 4201 aagcccgacg acccctgtcc tgaaggctgg cacactttcc cctccagcct ttcttgttat 4261 aaggtgttcc atatagaaag aatcgtaaga aagaggaact gggaagaagc cgaaaggttc 4321 tgccaagccc ttggagctca cctacccagc ttcagtcgta gagaggaaat taaggacttt 4381 gtgcatttgt taaaggacca gttcagtggg cagcgttggt tgtggattgg tctgaataag 4441 agaagccctg atttacaagg gtcctggcag tggagtgacc ggacaccagt gtctgctgtg 4501 atgatggagc cggagtttca acaggatttt gacatcagag actgtgctgc catcaaggtc 4561 cttgatgtac cttggcgaag agtctggcat ctctatgagg acaaggacta tgcttactgg 4621 aaaccttttg cttgtgatgc caagcttgag tgggtgtgcc agattccaaa aggtagcact 4681 ccccagatgc cagactggta taatccagag cgcactggaa ttcatgggcc cccagttata 4741 attgaaggaa gtgaatactg gtttgttgct gatccccact taaactacga agaagccgtc 4801 ttatactgtg ctagcaatca cagctttctt gccacgataa catcgttcac aggactaaaa 4861 gctatcaaaa acaaactagc aaatatttct ggcgaggaac agaagtggtg ggtgaaaacg 4921 agtgagaatc caattgatcg ttactttcta ggctcgcgcc gccgcctgtg gcaccatttc 4981 cccatgacgt ttggagatga atgtttgcac atgtcagcca agacgtggct tgttgactta 5041 agtaaacgag cggactgtaa tgccaagttg cccttcatct gtgaaagata caatgtctct 5101 tcattagaga aatacagccc agatcctgca gccaaagtac agtgcactga gaagtggatt 5161 ccttttcaaa ataagtgctt cctaaaggtc aactctgggc ccgttacgtt ttctcaagca 5221 agcggcattt gtcattccta cggcggcacc cttccttccg tgctgagccg gggtgaacaa 5281 gatttcatta tatccttgct tcctgaaatg gaagctagtc tatggattgg tctgcgctgg 5341 actgcctacg aaaggataaa cagatggaca gacaacagag agctgaccta cagcaacttt 5401 cacccactgc tggtcggtcg gaggctgagc ataccaacga atttctttga tgatgagtcc 5461 cacttccact gcgccttgat tcttaatctc aaaaagtcac cgcttactgg gacctggaat 5521 tttacttcct gttcagaacg acactctctg tctctctgtc aaaaatactc agagactgaa 5581 gacggacagc cctgggagaa cacttcaaaa acagtgaagt atctaaataa cctatacaaa 5641 atcatctcga agcccctgac gtggcacggc gctctgaagg agtgcatgaa agagaagatg 5701 aggttggtga gcatcacaga cccttaccag caggccttcc tcgcagtgca ggccaccctg 5761 cgcaacagct ccttctggat cggactctcc agtcaagatg atgaactcaa ctttggttgg 5821 tcagatggga aacgtcttca atttagtaac tgggctggaa gcaatgagca acttgatgac 5881 tgcgtgatat tagacacaga tggattctgg aaaacagctg actgtgatga taaccagcct 5941 ggcgccattt gctactatcc aggaaatgag actgaggagg aggtcagagc actggacact 6001 gctaaatgcc cgtctcctgt acagagcacc ccatggatac cattccagaa ctcctgctac 6061 aatttcatga ttaccaacaa caggcataag acagtcacac cggaggaagt gcagtccacg 6121 tgcgagaagc tgcattcgaa agcacacagt ctgagcattc ggaatgagga ggagaatacc 6181 tttgttgtgg aacagcttct gtacttcaat tatattgcct catgggtcat gttaggaata 6241 acctatgaaa acaattcttt gatgtggttt gataaaactg cattgtccta cacacactgg 6301 agaacgggaa gaccaactgt gaaaaatggc aaatttttgg ctggtctaag tactgatgga 6361 ttctgggata ttcagtcttt caatgttatt gaagaaacac ttcattttta ccagcacagt 6421 atttctgctt gtaaaattga aatggttgac tatgaggaca aacacaatgg caccctgcca 6481 cagttcattc catataagga cggcgtctac agcgttattc agaagaaggt gacgtggtat 6541 gaagcattga acgcgtgctc tcaaagtggg ggagagttgg ccagtgttca caacccaaat 6601 gggaagctct ttctggaaga cattgtgaac cgtgacggat tccctctctg ggttgggctc 6661 tcaagtcatg atggaagcga atcgagtttc gaatggtccg atggcagagc atttgactat 6721 gtcccatggc agagcctaca atctcccgga gactgtgtcg tcttatatcc aaaaggaatt 6781 tggagacgtg aaaaatgcct gtctgttaag gatggtgcta tttgttacaa gcctacaaaa 6841 gataaaaagc tgatctttca tgtaaaatca tcaaaatgtc cagtggcaaa gagggatggt 6901 ccccagtggg tccagtatgg gggccactgt tacgcttcgg accaggtact gcacagcttc 6961 tcagaggcca aacaagtgtg tcaagagctt gatcattcgg caactgttgt caccatagca 7021 gatgaaaatg agaataagtt tgtgagcaga ctgatgaggg agaactataa tattactatg 7081 agagtttggc ttggcctgtc tcagcattca ctcgatcagt cttggagttg gctcgatgga 7141 ttagatgtga catttgtcaa atgggaaaat aaaactaagg atggtgatgg gaaatgtagc 7201 attttaatag cttcaaatga aacctggaga aaagtccatt gctcacgtgg ctatgcaaga 7261 gctgtctgca aaattcctct gagcccggac tacacaggca tagccatcct gtttgccgtg 7321 ctgtgcctct tagggctcat cagcttggcg atttggttcc tcttgcaacg atcccatatc 7381 cgctggaccg gcttctcctc ggttcggtat gaacatggaa ccaacgaaga cgaggtgatg 7441 ctcccttctt tccacgacTC TAGATACCCA TACGACGTAC CTGATTATGC AACTTATCCT 7501 TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC AGGATCCgag 7561 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc ttccggaatg 7621 gagagcgacg agagcggcct ggtgagcaag ggcgaggagg ataacatggc catcatcaag 7681 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 7741 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 7801 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 7861 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 7921 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 7981 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 8041 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 8101 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 8161 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 8221 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 8281 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 8341 tacaagtagg gatccgcggc cgcaaggatc tgcgatcgct ccggtgcccg tcagtgggca 8401 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 8461 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 8521 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 8581 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 8641 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 8701 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 8761 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 8821 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 8881 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 8941 ggcctgatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa 9001 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga 9061 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa 9121 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc 9181 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc 9241 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg 9301 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac 9361 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac 9421 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa 9481 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc 9541 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga 9601 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta 9661 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt 9721 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag 9781 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc 9841 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc 9901 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa 9961 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa 10021 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc 10081 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg 10141 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta 10201 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg 10261 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg 10321 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt 10381 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc 10441 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt 10501 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc 10561 ctcataaagg ccaagaaggg cggaaagatc gccgtgagat cccgcgctca gtcgtccaat 10621 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 10681 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 10741 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 10801 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 10861 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 10921 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 10981 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 11041 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 11101 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 11161 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 11221 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 11281 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 11341 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 11401 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 11461 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 11521 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 11581 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 11641 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 11701 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 11761 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 11821 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt 11881 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca 11941 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 12001 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 12061 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 12121 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 12181 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 12241 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 12301 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 12361 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 12421 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 12481 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 12541 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt 12601 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 12661 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 12721 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 12781 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 12841 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 12901 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 12961 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 13021 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 13081 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 13141 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 13201 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 13261 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 13321 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 13381 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 13441 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 13501 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 13561 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 13621 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 13681 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 13741 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 13801 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 13861 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 13921 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 13981 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 14041 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 14101 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 14161 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 14221 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 14281 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 14341 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 14401 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 14461 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 14521 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 14581 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 14641 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 14701 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 14761 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 14821 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 14881 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 14941 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 15001 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 15061 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 15121 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 15181 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.