pLV3-CMV-VCP-3×FLAG-CopGFP-Pur

pLV3-CMV-VCP-3×FLAG-CopGFP-Puro plasmid

PVT111405

pLV3-CMV-VCP-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-VCP(human)-3×FLAG-CopGFP-Puro
Alias: NM_007126.5;CDC48; FTDALS6; p97; TERA
Gene lenght: 2418
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-VCP-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-VCP-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-VCP-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cttctggagc cgattcaaaa 2341 ggtgatgacc tatcaacagc cattctcaaa cagaagaacc gtcccaatcg gttaattgtt 2401 gatgaagcca tcaatgagga caacagtgtg gtgtccttgt cccagcccaa gatggatgaa 2461 ttgcagttgt tccgaggtga cacagtgttg ctgaaaggaa agaagagacg agaagctgtt 2521 tgcatcgtcc tttctgatga tacttgttct gatgagaaga ttcggatgaa tagagttgtt 2581 cggaataacc ttcgtgtacg cctaggggat gtcatcagca tccagccatg ccctgatgtg 2641 aagtacggca aacgtatcca tgtgctgccc attgatgaca cagtggaagg cattactggt 2701 aatctcttcg aggtatacct taagccgtac ttcctggaag cgtatcgacc catccggaaa 2761 ggagacattt ttcttgtccg tggtgggatg cgtgctgtgg agttcaaagt ggtggaaaca 2821 gatcctagcc cttattgcat tgttgctcca gacacagtga tccactgcga aggggagcct 2881 atcaaacgag aggatgagga agagtccttg aatgaagtag ggtatgatga cattggtggc 2941 tgcaggaagc agctagctca gataaaggag atggtggaac tgcccctgag acatcctgcc 3001 ctctttaagg caattggtgt gaagcctcct agaggaatcc tgctttacgg acctcctgga 3061 acaggaaaga ccctgattgc tcgagctgta gcaaatgaga ctggagcctt cttcttcttg 3121 atcaatggtc ctgagatcat gagcaaattg gctggtgagt ctgagagcaa ccttcgtaaa 3181 gcctttgagg aggctgagaa gaatgctcct gccatcatct tcattgatga gctagatgcc 3241 atcgctccca aaagagagaa aactcatggc gaggtggagc ggcgcattgt atcacagttg 3301 ttgaccctca tggatggcct aaagcagagg gcacatgtga ttgttatggc agcaaccaac 3361 agacccaaca gcattgaccc agctctacgg cgatttggtc gctttgacag ggaggtagat 3421 attggaattc ctgatgctac aggacgctta gagattcttc agatccatac caagaacatg 3481 aagctggcag atgatgtgga cctggaacag gtagccaatg agactcacgg gcatgtgggt 3541 gctgacttag cagccctgtg ctcagaggct gctctgcaag ccatccgcaa gaagatggat 3601 ctcattgacc tagaggatga gaccattgat gccgaggtca tgaactctct agcagttact 3661 atggatgact tccggtgggc cttgagccag agtaacccat cagcactgcg ggaaaccgtg 3721 gtagaggtgc cacaggtaac ctgggaagac atcgggggcc tagaggatgt caaacgtgag 3781 ctacaggagc tggtccagta tcctgtggag cacccagaca aattcctgaa gtttggcatg 3841 acaccttcca agggagttct gttctatgga cctcctggct gtgggaaaac tttgttggcc 3901 aaagccattg ctaatgaatg ccaggccaac ttcatctcca tcaagggtcc tgagctgctc 3961 accatgtggt ttggggagtc tgaggccaat gtcagagaaa tctttgacaa ggcccgccaa 4021 gctgccccct gtgtgctatt ctttgatgag ctggattcga ttgccaaggc tcgtggaggt 4081 aacattggag atggtggtgg ggctgctgac cgagtcatca accagatcct gacagaaatg 4141 gatggcatgt ccacaaaaaa aaatgtgttc atcattggcg ctaccaaccg gcctgacatc 4201 attgatcctg ccatcctcag acctggccgt cttgatcagc tcatctacat cccacttcct 4261 gatgagaagt cccgtgttgc catcctcaag gctaacctgc gcaagtcccc agttgccaag 4321 gatgtggact tggagttcct ggctaaaatg actaatggct tctctggagc tgacctgaca 4381 gagatttgcc agcgtgcttg caagctggcc atccgtgaat ccatcgagag tgagattagg 4441 cgagaacgag agaggcagac aaacccatca gccatggagg tagaagagga tgatccagtg 4501 cctgagatcc gtcgagatca ctttgaagaa gccatgcgct ttgcgcgccg ttctgtcagt 4561 gacaatgaca ttcggaagta tgagatgttt gcccagaccc ttcagcagag tcggggcttt 4621 ggcagcttca gattcccttc agggaaccag ggtggagctg gccccagtca gggcagtgga 4681 ggcggcacag gtggcagtgt atacacagaa gacaatgatg atgacctgta tggcggatcc 4741 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 4801 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 4861 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 4921 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 4981 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 5041 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 5101 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 5161 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 5221 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 5281 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 5341 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 5401 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 5461 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 5521 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 5581 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 5641 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 5701 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 5761 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 5821 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 5881 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 5941 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 6001 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 6061 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 6121 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 6181 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 6241 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 6301 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 6361 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 6421 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 6481 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 6541 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 6601 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 6661 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 6721 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 6781 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6841 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6901 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6961 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 7021 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 7081 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 7141 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 7201 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 7261 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 7321 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 7381 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 7441 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 7501 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 7561 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 7621 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 7681 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 7741 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 7801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 7861 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 7921 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 7981 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 8041 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 8101 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 8161 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 8221 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 8281 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 8341 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 8401 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 8461 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 8521 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 8581 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 8641 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 8701 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 8761 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 8821 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 8881 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 8941 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 9001 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 9061 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 9121 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 9181 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 9241 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 9301 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 9361 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 9421 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 9481 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 9541 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 9601 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 9661 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 9721 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 9781 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 9841 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 9901 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 9961 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 10021 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 10081 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 10141 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 10201 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 10261 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 10321 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 10381 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 10441 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 10501 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 10561 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 10621 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 10681 ttgtaaaacg acggccagtg ccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.