pLV3-CMV-Acsl4-Fluc-Puro plasm

pLV3-CMV-Acsl4-Fluc-Puro plasmid

PVT111396

pLV3-CMV-Acsl4-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-Acsl4(mouse)-Fluc-Puro
Alias: NM_207625.2;9430020A05Rik; ACS4; Facl4; Lacs4
Gene lenght: 2136
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Acsl4-Fluc-Puro plasmid Description

pLV3-CMV-Acsl4-Fluc-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Acsl4-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgaacctt aagctaaatg tgctcaccat tatattgctg 2341 cctgtccact tgttaataac aatatacagt gcccttatat ttattccatg gtattttctt 2401 accaatgcca agaagaaaaa cgctatggca aagagaataa aagctaagcc cacttcagac 2461 aaacctggaa gtccatatcg ctctgtcaca cacttcgact cactagctgt catagacatc 2521 cctggagcag atactctgga taaattattt gaccatgctg tagccaaatt tgggaagaag 2581 gacagccttg gaacccggga gatcctgagt gaagaaaatg aaatgcagcc aaatggaaag 2641 gtttttaaga agttaattct tgggaattat aaatggataa actatcttga agtgaactgc 2701 agagtgaata actttggaag tggcctcact gcattgggac tgaaaccaaa gaacaccatt 2761 gccattttct gtgagaccag ggcagagtgg atgattgcag cacagacttg ctttaagtac 2821 aactttccac ttgtgacttt atatgccaca cttggcagag aagctgtagt tcatggatta 2881 aatgaatctg aggcttccta tctgattact agtgttgagc ttctggaaag caaactgaag 2941 gcggccttag tagatatcaa ttgtgttaaa catatcattt atgtggataa taagactatc 3001 aatagagcag agtaccctga ggggcttgaa attcacagca tgcaatcagt agaggagctg 3061 ggagccaagc cagaaaactt gagcgttcct ccaagtagac caaccccttc agacatggcc 3121 attgtcatgt acaccagtgg ttctacgggc cgccccaagg gagtgatgat gcatcatagc 3181 aatttgattg ctggaatgac aggccagtgt gaacgtatcc ctggactagg accgaaggac 3241 acatatattg gctacttacc tttggctcat gtgctggaac tgacagcaga gatatcatgc 3301 tttacctatg gctgtaggat tggatactct tcacccctta cactgtctga ccagtccagc 3361 aaaatcaaga agggaagcaa gggtgattgt actgtactga aacccacact tatggccgct 3421 gttccggaaa tcatggatag aatttataag aatgttatga gcaaggttca agagatgaat 3481 tatgttcaga aaactctatt taaaatcggg tatgattaca aattagagca aatcaagaaa 3541 ggctatgacg cccctctttg taatctgata ctgtttaaaa aggtgaaggc tctgctggga 3601 gggaatgtcc gcatgatgct gtctggcggc gcgccactgt cccctcagac acaccgattc 3661 atgaatgtct gcttctgctg ccccattggt cagggatatg ggctgacaga atcatgtggt 3721 gctggaacag ttactgaagt tactgactac actactggaa gagttggagc tcctcttatt 3781 tgctgtgaaa ttaaactgaa agactggcag gaaggtggtt atacagttca tgataagccg 3841 aaccccagag gtgagattgt gatcggtggc cagaatatct ccatgggata ttttaaaaac 3901 gaagagaaaa cagcagaaga ttattgtgtt gatgaaaatg gacaaaggtg gttttgcact 3961 ggcgatattg gagaattcca tcctgatgga tgcttacaga ttatagatcg taagaaagat 4021 ctggtaaagt tacaagcagg agaatatgta tctcttggga aagtagaagc tgcactgaag 4081 aattgtccac tgatcgacaa catctgtgct tttgccaaaa gtgaccagtc ctatgtgatc 4141 agttttgtgg ttcctaacca gaaaaagttg actcttttgg cacaacagaa gggggtagaa 4201 ggatcttggg ttgatatttg caataatccc gccatggaag ctgaaatact gaaagaaatt 4261 cgagaagctg caaatgccat gaaattggag cgatttgaaa ttccgatcaa ggttcggtta 4321 agcccagagc catggacccc cgagactggt ttggtaacag atgccttcaa gctgaaaagg 4381 aaggagttga agaaccatta tctcaaagac attgagcgaa tgtatggggg caaataagga 4441 tccgcggccg caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4501 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4561 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4621 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4681 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4741 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4801 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4861 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4921 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4981 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgatggaa 5041 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct 5101 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt 5161 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg 5221 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt 5281 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt 5341 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag 5401 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag 5461 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag 5521 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg 5581 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact 5641 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc 5701 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca 5761 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc 5821 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt 5881 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac 5941 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa 6001 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc 6061 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc 6121 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg 6181 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta 6241 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac 6301 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt 6361 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc 6421 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc 6481 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc 6541 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta 6601 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc 6661 aagaagggcg gaaagatcgc cgtgagatcc cgcgctcagt cgtccaattc tgccgtggac 6721 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 6781 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 6841 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 6901 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 6961 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 7021 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 7081 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 7141 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 7201 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 7261 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 7321 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 7381 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 7441 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 7501 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 7561 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 7621 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 7681 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 7741 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 7801 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 7861 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 7921 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt 7981 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 8041 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa 8101 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 8161 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 8221 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 8281 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 8341 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 8401 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 8461 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 8521 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 8581 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 8641 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 8701 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 8761 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 8821 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 8881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 8941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 9001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 9061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 9121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 9181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 9241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 9301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 9361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 9421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 9481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 9541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 9601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 9661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 9721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 9781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 9841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 9901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 9961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 10021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 10081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 10141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 10201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 10261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 10321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 10381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 10441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 10501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 10561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 10621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 10681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 10741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 10801 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 10861 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 10921 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 10981 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 11041 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 11101 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 11161 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 11221 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 11281 gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.