pLV2-CMV-mNeonGreen-Puro plasm

pLV2-CMV-mNeonGreen-Puro plasmid

PVT103938

pLV2-CMV-mNeonGreen-Puro plasmid Information

Plasmid name: pLV2-CMV-mNeonGreen-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-mNeonGreen-Puro plasmid Description

pLV2-CMV-mNeonGreen-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-mNeonGreen-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggtgag caagggcgag gaggataaca tggcctctct 2881 cccagcgaca catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt 2941 gggtcagggc accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa 3001 gggtgacctc cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca 3061 gtacctgccc taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg 3121 ataccaagtc catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg 3181 ctacacctac gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc 3241 tgctgacggt cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa 3301 gacttacccc aacgacaaaa ccatcatcag tacctttaag tggagttaca ccactggaaa 3361 tggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc 3421 taactatctg aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa 3481 gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga 3541 gctgtacaag tgaggatccc gcgactctag ataattctac cgggtagggg aggcgctttt 3601 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag 3661 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt 3721 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 3781 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 3841 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 3901 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct 3961 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg 4021 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc 4081 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc 4141 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 4201 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 4261 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 4321 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 4381 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 4441 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 4501 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 4561 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 4621 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 4681 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg 4741 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4801 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4861 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4921 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4981 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5041 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5101 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 5161 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 5221 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 5281 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat 5341 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 5401 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 5461 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 5521 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 5581 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 5641 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 5701 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 5761 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 5821 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 5881 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 5941 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 6001 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6061 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6121 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6181 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt 6241 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6301 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 6361 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 6421 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 6481 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6541 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 6601 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 6661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 6721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 6781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 7141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7201 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7381 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7441 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 7501 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7561 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 7621 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7681 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 7741 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 7801 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 7861 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7921 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7981 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8041 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 8101 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 8161 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 8221 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 8281 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 8341 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8401 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca 8461 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 8521 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 8581 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 8641 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca 8701 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt 8761 gtatttgtta aatatgtact acaaacttag tagt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.