pLV3-CMV-MCS-3×FLAG-EF1a-CopGF

pLV3-CMV-MCS-3×FLAG-EF1a-CopGFP-Blast plasmid

PVT111368

pLV3-CMV-MCS-3×FLAG-EF1a-CopGFP-Blast plasmid Information

Plasmid name: pLV3-CMV-MCS-3×FLAG-EF1a-CopGFP-Blast
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-3×FLAG-EF1a-CopGFP-Blast plasmid Description

pLV3-CMV-MCS-3×FLAG-EF1a-CopGFP-Blast It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-MCS-3×FLAG-EF1a-CopGFP-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc ttcgaaaccg gtCTCGAGGA CTACAAAGAC 2341 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGTAGgga 2401 tccCTGCGAT ATCCAGCgcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 2461 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 2521 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 2581 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 2641 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 2701 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 2761 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 2821 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 2881 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 2941 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 3001 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 3061 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 3121 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 3181 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 3241 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 3301 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 3361 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 3421 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 3481 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 3541 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 3601 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 3661 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 3721 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 3781 gagaatcccg gccctATGGC CAAGCCTTTG TCTCAAGAAG AATCCACCCT CATTGAAAGA 3841 GCAACGGCTA CAATCAACAG CATCCCCATC TCTGAAGACT ACAGCGTCGC CAGCGCAGCT 3901 CTCTCTAGCG ACGGCCGCAT CTTCACTGGT GTCAATGTAT ATCATTTTAC TGGGGGACCT 3961 TGTGCAGAAC TCGTGGTGCT GGGCACTGCT GCTGCTGCGG CAGCTGGCAA CCTGACTTGT 4021 ATCGTCGCGA TCGGAAATGA GAACAGGGGC ATCTTGAGCC CCTGCGGACG GTGCCGACAG 4081 GTGCTTCTCG ATCTGCATCC TGGGATCAAA GCCATAGTGA AGGACAGTGA TGGACAGCCG 4141 ACGGCAGTTG GGATTCGTGA ATTGCTGCCC TCTGGTTATG TGTGGGAGGG CTAAGTCGAC 4201 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 4261 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 4321 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 4381 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 4441 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 4501 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 4561 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 4621 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 4681 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 4741 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCc tggtaccttt 4801 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 4861 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc 4921 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 4981 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 5041 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 5101 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 5161 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 5221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 5281 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat 5341 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 5401 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 5461 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 5521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 5581 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 5641 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5701 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 6001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 6061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6301 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 6361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 6601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 6661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 6721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 6781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 6961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 7021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 7081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 7141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 7201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 7261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 7321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 7381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 7441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 7501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 7561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 7621 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 7681 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 7741 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 7801 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 7861 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 7921 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 7981 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 8041 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 8101 aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.