pTac-GST-CDC42 plasmid

pTac-GST-CDC42 plasmid

PVT111073

pTac-GST-CDC42 plasmid Information

Plasmid name: pTac-GST-CDC42(human)
Alias: NM_001039802.2;CDC42Hs; G25K; TKS
Gene lenght: 573
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F1
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-CDC42 plasmid Description

pTac-GST-CDC42 Plasmid suitable for gene expression in Escherichia coli.

pTac-GST-CDC42 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatgca gacaattaag tgtgttgttg 961 tgggcgatgg tgctgttggt aaaacatgtc tcctgatatc ctacacaaca aacaaatttc 1021 catcggaata tgtaccgact gtttttgaca actatgcagt cacagttatg attggtggag 1081 aaccatatac tcttggactt tttgatactg cagggcaaga ggattatgac agattacgac 1141 cgctgagtta tccacaaaca gatgtatttc tagtctgttt ttcagtggtc tctccatctt 1201 catttgaaaa cgtgaaagaa aagtgggtgc ctgagataac tcaccactgt ccaaagactc 1261 ctttcttgct tgttgggact caaattgatc tcagagatga cccctctact attgagaaac 1321 ttgccaagaa caaacagaag cctatcactc cagagactgc tgaaaagctg gcccgtgacc 1381 tgaaggctgt caagtatgtg gagtgttctg cacttacaca gaaaggccta aagaatgtat 1441 ttgacgaagc aatattggct gccctggagc ctccagaacc gaagaagagc cgcaggtgtg 1501 tgctgctaTA Gctcgagcgg ccgcatcgtg actgactgac gatctgcctc gcgcgtttcg 1561 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 1621 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 1681 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct tgaagacgaa 1741 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 1801 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 1861 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 1921 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 1981 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 2041 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 2101 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 2161 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt 2221 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 2281 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 2341 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 2401 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 2461 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac 2521 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 2581 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 2641 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 2701 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 2761 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 2821 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 2881 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 2941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 3001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 3061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 3121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 3181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 3241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 3301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 3361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 3421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 3481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 3541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 3601 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 3661 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 3721 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 3781 cacaccgcat aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat 3841 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg 3901 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc 3961 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 4021 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 4081 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 4141 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 4201 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 4261 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 4321 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 4381 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 4441 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 4501 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 4561 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 4621 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct 4681 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 4741 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 4801 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 4861 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 4921 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 4981 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 5041 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacggattc 5101 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 5161 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 5221 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc 5281 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt 5341 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc 5401 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac 5461 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt 5521 tggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.