pCMV-EGFP-Aebp1-3×HA-Neo plasm

pCMV-EGFP-Aebp1-3×HA-Neo plasmid

PVT111053

pCMV-EGFP-Aebp1-3×HA-Neo plasmid Information

Plasmid name: pCMV-EGFP-Aebp1(mouse)-3×HA-Neo
Alias: NM_001291857.2;ACLP
Gene lenght: 3387
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pCMV-EGFP-Aebp1-3×HA-Neo plasmid Description

pCMV-EGFP-Aebp1-3×HA-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-EGFP-Aebp1-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagG CGGCGGAGGG 1621 AGTGGAGGAG GGGGAAGCgg aggcggaggg agtAAGCTTa tggctccagt gcgcaccgca 1681 tccctgctct gcggcctcct ggcactgctg acgctgtgcc ctgaggggaa cccacagacg 1741 gtgctgacgg acgacgagat cgaggagttc ctcgaaggct tcctttcgga gttggagacc 1801 cagtccccgc cccgggaaga cgacgtggaa gtccagccgc ttcccgaacc cacccagcgt 1861 ccccgcaaat ccaaggcagg gggcaagcag cgggcagatg tagaagtccc tccagaaaaa 1921 aacaaagaca aagagaagaa aggaaagaag gacaaaggcc ccaaagccac aaaacccctg 1981 gagggctcta ccaggcccac caagaaacca aaggagaagc cacccaaggc caccaagaag 2041 cccaaggaga aaccacccaa ggccaccaag aagcccaagg agaagccacc caaggccacc 2101 aagaagccta aggagaagcc acccaaggcc actaagaggc cctcggcagg aaagaagttc 2161 tcaactgtgg cccccttgga aacgctggat cggttactcc cctcaccctc caaccccagc 2221 gcccaggagc taccgcagaa gagagacaca cccttcccaa atgcctggca aggtcaagga 2281 gaagagaccc aggtggaggc caagcagccc cggccagagc cagaggagga gactgagatg 2341 cccacactgg actacaatga ccagatagag aaggaggatt acgaggattt tgagtacatc 2401 cgtcgccaga agcagcccag gccaacaccc agcaggagga ggctctggcc agagcgccct 2461 gaggagaaga ctgaagagcc agaggaaagg aaggaagtcg agccacctct gaagcccctg 2521 ctgcctccgg actatgggga tagctacgtg atccccaact atgatgactt ggactattat 2581 ttcccccacc ctccaccgca gaagcctgat gttggacaag aggtggatga ggaaaaggaa 2641 gagatgaaga agcccaaaaa ggagggtagt agccccaagg aggacacaga ggacaagtgg 2701 accgtggaga aaaacaagga ccacaaagca gggccccgga agggtgagga gctggaggag 2761 gagtgggcgc cagtggagaa aatcaagtgc ccacctattg ggatggagtc acaccgcatt 2821 gaggacaacc agatccgtgc ctcctccatg ctgcgccacg gcctcggagc ccagcggggc 2881 cggctcaaca tgcaggctgg tgccaatgaa gatgactact atgacggggc atggtgtgct 2941 gaggacgagt cgcagaccca gtggatcgag gtggacaccc gaaggacaac tcggttcacg 3001 ggcgtcatca ctcagggccg tgactccagc atccatgacg acttcgtgac taccttcttt 3061 gtgggcttca gcaatgacag ccagacctgg gtgatgtaca ccaatggcta cgaggaaatg 3121 accttctatg gaaatgtgga caaggacaca cctgtgctga gcgagctccc tgagccagtt 3181 gtggcccgtt tcatccgcat ctatccactc acctggaacg gtagcctgtg catgcgcctg 3241 gaggtgctag gctgccccgt gacccctgtc tacagctact acgcacagaa tgaggtggta 3301 actactgaca gcctggactt ccggcaccac agctacaagg acatgcgcca gctgatgaag 3361 gctgtcaatg aggagtgccc cacaatcact cgcacataca gcctgggcaa gagttcacga 3421 gggctcaaga tctacgcaat ggaaatctca gacaaccctg gggatcatga actgggggag 3481 cccgagttcc gctacacagc cgggatccac ggcaatgagg tgctaggccg agagctcctg 3541 ctcctgctca tgcaatacct atgccaggag taccgcgatg ggaacccgag agtgcgcaac 3601 ctggtgcagg acacacgcat ccacctggtg ccctcgctga accctgatgg ctatgaggtg 3661 gcagcgcaga tgggctcaga gtttgggaac tgggcactgg ggctgtggac tgaggagggc 3721 tttgacatct tcgaggactt cccagatctc aactctgtgc tctgggcagc tgaggagaag 3781 aaatgggtcc cctacagggt cccaaacaat aacttgccaa tccctgaacg ttacctgtcc 3841 ccagatgcca cggtctccac agaagtccgg gccattattt cctggatgga gaagaacccc 3901 tttgtgctgg gtgcaaatct gaacggtggt gagcggcttg tgtcttatcc ctatgacatg 3961 gcccggacac ctagccagga gcagctgttg gccgaggcac tggcagctgc ccgcggagaa 4021 gatgatgacg gggtgtctga ggcccaggag actccagatc acgctatttt ccgctggctg 4081 gccatctcat ttgcctccgc ccatctcacc atgacggagc cctaccgggg agggtgccag 4141 gcccaggact acaccagcgg catgggcatt gtcaacgggg ccaagtggaa tcctcgctct 4201 gggactttca atgactttag ctacctgcac acaaactgtc tggagctctc cgtatacctg 4261 ggctgtgaca agttccccca cgagagtgag ctaccccgag aatgggagaa caacaaagaa 4321 gcgctgctca ccttcatgga gcaggtgcac cgtggcatta agggtgtggt gacagatgag 4381 caaggcatcc ccattgccaa tgccaccatc tctgtgagtg gcatcaacca tggtgtgaag 4441 acagcaagtg gaggtgacta ctggcgcatt ctgaacccgg gtgagtaccg tgtgacagct 4501 cacgcagagg gctacacctc aagtgccaag atctgcaatg tggactacga tattggggcc 4561 actcagtgca acttcatcct ggctcgatcc aactggaagc gcattcggga gatcttggct 4621 atgaacggga accgtcccat tctccgagtt gacccctcac gacccatgac cccccagcag 4681 cggcgcatgc agcagcgccg tctacagtac cggctccgca tgagggaaca gatgcgactg 4741 cgtcgcctca attctaccgc aggccctgcc acaagcccca ctcctgccct tatgcctccc 4801 ccttccccta caccagccat taccttgagg ccctgggaag ttctacccac taccactgca 4861 ggctgggagg agtcagagac tgagacctat acagaagtag tgacagagtt tgagacagag 4921 tatgggactg acctagaggt ggaagagata gaggaggagg aggaggagga ggaggaagag 4981 atggacacag gccttacatt tccactcaca acagtggaga cctacacagt gaactttggg 5041 gacttcGGTA CCTACCCATA CGACGTACCT GATTATGCAA CTTATCCTTA TGATGTACCT 5101 GATTATGCTA GCTATCCTTA TGATGTCCCA GACTACGCAT AGGATCCACT AGTCCAGTGT 5161 GGTGGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC GAGTCTAGAG GGCCCGTTTA 5221 AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 5281 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 5341 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 5401 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC 5461 TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG 5521 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 5581 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 5641 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 5701 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 5761 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 5821 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 5881 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA 5941 ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 6001 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 6061 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 6121 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 6181 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 6241 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 6301 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 6361 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 6421 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 6481 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 6541 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 6601 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 6661 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 6721 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 6781 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 6841 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 6901 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 6961 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 7021 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 7081 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 7141 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 7201 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 7261 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 7321 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 7381 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 7441 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 7501 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 7561 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 7621 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 7681 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 7741 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 7801 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 7861 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 7921 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 7981 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 8041 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 8101 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 8161 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 8221 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 8281 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 8341 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 8401 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 8461 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 8521 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 8581 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 8641 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 8701 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 8761 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 8821 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 8881 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 8941 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 9001 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 9061 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 9121 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 9181 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 9241 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 9301 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 9361 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 9421 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 9481 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 9541 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 9601 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC 9661 CGATCCCCTA TGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.