pCMV-ASPN-3×HA-EGFP-Neo plasmi

pCMV-ASPN-3×HA-EGFP-Neo plasmid

PVT110425

pCMV-ASPN-3×HA-EGFP-Neo plasmid Information

Plasmid name: pCMV-ASPN(human)-3×HA-EGFP-Neo
Alias: NM_017680.6;OS3; PLAP-1; PLAP1; SLRR1C
Gene lenght: 1137
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ASPN-3×HA-EGFP-Neo plasmid Description

pCMV-ASPN-3×HA-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-ASPN-3×HA-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatga aggagtatgt gctcctatta ttcctggctt 961 tgtgctctgc caaacccttc tttagccctt cacacatcgc actgaagaat atgatgctga 1021 aggatatgga agacacagat gatgatgatg atgatgatga tgatgatgat gatgatgagg 1081 acaactctct ttttccaaca agagagccaa gaagccattt ttttccattt gatctgtttc 1141 caatgtgtcc atttggatgt cagtgctatt cacgagttgt acattgctca gatttaggtt 1201 tgacctcagt cccaaccaac attccatttg atactcgaat gcttgatctt caaaacaata 1261 aaattaagga aatcaaagaa aatgatttta aaggactcac ttcactttat ggtctgatcc 1321 tgaacaacaa caagctaacg aagattcacc caaaagcctt tctaaccaca aagaagttgc 1381 gaaggctgta tctgtcccac aatcaactaa gtgaaatacc acttaatctt cccaaatcat 1441 tagcagaact cagaattcat gaaaataaag ttaagaaaat acaaaaggac acattcaaag 1501 gaatgaatgc tttacacgtt ttggaaatga gtgcaaaccc tcttgataat aatgggatag 1561 agccaggggc atttgaaggg gtgacggtgt tccatatcag aattgcagaa gcaaaactga 1621 cctcagttcc taaaggctta ccaccaactt tattggagct tcacttagat tataataaaa 1681 tttcaacagt ggaacttgag gattttaaac gatacaaaga actacaaagg ctgggcctag 1741 gaaacaacaa aatcacagat atcgaaaatg ggagtcttgc taacatacca cgtgtgagag 1801 aaatacattt ggaaaacaat aaactaaaaa aaatcccttc aggattacca gagttgaaat 1861 acctccagat aatcttcctt cattctaatt caattgcaag agtgggagta aatgacttct 1921 gtccaacagt gccaaagatg aagaaatctt tatacagtgc aataagttta ttcaacaacc 1981 cggtgaaata ctgggaaatg caacctgcaa catttcgttg tgttttgagc agaatgagtg 2041 ttcagcttgg gaactttgga atgAAGCTTT ACCCATACGA CGTACCTGAT TATGCAACTT 2101 ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC TACGCActcg 2161 agggcggtgg aggaagcggc ggaggtggat ctatggtgag caagggcgag gagctgttca 2221 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 2281 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 2341 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 2401 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 2461 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 2521 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 2581 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 2641 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 2701 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 2761 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 2821 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 2881 tcactctcgg catggacgag ctgtacaagt agtctagagg gcccgtttaa acccgctgat 2941 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 3001 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 3061 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 3121 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 3181 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 3241 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 3301 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 3361 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 3421 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 3481 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 3541 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 3601 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 3661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 3721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 3781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 3841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 3901 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 3961 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 4021 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 4081 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 4141 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 4201 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 4261 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 4321 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 4381 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 4441 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 4501 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 4561 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 4621 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 4681 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 4741 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 4801 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 4861 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 4921 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 4981 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 5041 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 5101 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 5161 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 5221 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 5281 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 5341 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 5401 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 5461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 5521 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 5581 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 5701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 5761 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 5821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 5881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 5941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 6001 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 6061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 6121 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 6181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 6241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 6301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 6481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 6541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 6601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 6661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 6721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 6781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 6841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 6901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 6961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 7141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 7201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 7321 acatttcccc gaaaagtgcc acctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.