pCYP7B1-Fluc-SV40-hRluc-Neo pl

pCYP7B1-Fluc-SV40-hRluc-Neo plasmid

PVT110422

pCYP7B1-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pCYP7B1(human)-Fluc-SV40-hRluc-Neo
Alias:
Gene lenght: 2001
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc,Rluc
Promoter: CYP7B1
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc（special construction）
Reverse primer:
Copy number: High
Induction:

pCYP7B1-Fluc-SV40-hRluc-Neo plasmid Description

pCYP7B1-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pCYP7B1-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattcC CAACCTCTTC AGCATAAAGC TCCCACCCCA AGCCCTCCTT TGAAGGGCCT 5161 GTCTCTAGTT TTGCCTGGAT GGCCACCTTG CAGGCTGTAA CCCTTTGGAA GAAATAAAGT 5221 CTCCTCTCTC CTTTTCAAAA CTTAAAAATT GTGAATTTTT ATTTTTATTT TATGTATTTA 5281 ACTTTTAAGT TTAGGACACA TGTGCAGGTT TGTTATATAG GTAAATTCAC ATCATGGGGG 5341 TTTGTTGTAC AGATTATTTC ATCACCAAGC TATTAAGCCC AGTAATTATT AGTCATTTTT 5401 TCTGATCGTC TCCCTCCTCA CCCACTCTGC CCTCCAGCAG GCCCCAGTAT CCGTTGTTCC 5461 CCTCTATGTG TCCATGTGTT CTCATCATTT AGCTCCCACT TATAAGTGAG AACATGCTGT 5521 ATTTGCTTTT CTGTTGCTAA GGATAATGGC CCCCAGCTCC ATCCATGTTC CTGCAAAGGA 5581 TATGATCTCA TTCTTTTTTA TGGCTGCATA GAATTTCATG GTGTATATAC CACATTTTCT 5641 TTATGCAGTC TACCACTGAT GGACATTTAG GTTGATTCCA TGTCTTTGCT ATTGTGAACA 5701 GTGCTGCAAT GAACATAAGC GTGCATGTGT CTTTATGACA GAGTGATTTA TATTCCTTTG 5761 GGTATATACA CAGTAATGGG ATTGCCAGAT CCTATGGTAG TTCTGTCTTT GAGGAACTAC 5821 CACACTGATT TCCAAAATAC ATGTGGCCAA CAAGCATATG AAAAAAAGCT CAATGACACT 5881 GATCATTAGA GAAATGCAAA TCAAAACTAC AATGGATTTT TTAAGTTAAT AATGTGAACA 5941 GGCTGAGTAT GAGGGACATG GTGGCCCAAG CTTGAGTATA TATATGGAAC CGTTCTGGCG 6001 AGGGGAGTTG TACAATGAAG ACAATTTGTG CAGGGCTTCT CATTGCAGGT GGAGGATATG 6061 ACTTCGTCAC AAAGACAACC ATTAAGAGTT TTGAACAGAG TAAAAGCTGG AGAGGTCTGT 6121 GTTTTAGGGA GGTGGAAATG GGGATGATGG ACTGGAAGGG TAAAAAACCA GAGATTGGTT 6181 TTGAAGCTGC TGTACCCATT CCAGAGAAGT GTTGCATTGA CAGAGCACAC AAGTCCTCGA 6241 ATAGGACCGT GGTTAGAACG GCCGATATTT ATCAGTGGGA ATGGTAAACA TTTATCAAGT 6301 GAGCAACCAA GTTCAGTTAC TTAATCTGAG TCTCATTATG TCTTCTATAA AATACACATT 6361 AAAAATAGAA AGTAAAAACG TGGGTAAAGT ACCCAGTGGA TGGTCATTAC TCAGAAAAAG 6421 GTATTTTAAA AGTTTCTCCC TTATTCTTTC TGAGTACAGC CTGTAGTACT TGAACCACTT 6481 CTCTTTCGTC CCCTACCACG CTGTTCTGCA ATCTCTTTTT ACCTGTCTCA CTCTCTTCCT 6541 GTACTGTGTG CCTCATCGGG ACTTCTAAAG GCTTACGGCC TAGGTCTTTG TATTCCGTGT 6601 CTCTGGCACG CGCCTCTGCA CACAGCAGGC CTGAACCAAA ACTTGTGAGC AAATAGGATG 6661 TAAGGGGGTG AAGGAGGTGG GCGCGAAACG GGTTCGAGGT CGCGCACTCC GCCGGTTTCT 6721 GCCGCCAGCC CAACCCCGCT GGGTCGTACC TAAGAGATCG CCGGTGTCAG AGCGCAGCGG 6781 GCACCTTCAC AAGCATCTTG AGGGACAAGC AGAGTCGCTC CTCCCGCAGG GCGCGGGCTA 6841 AGAAGTGAGG AACCCAAGGG GCTCCCGTCC AACTAAGAAG CGAAATCCCC CACCTCCAGG 6901 CCTTCAGGGC TGGTTCTTGG GAAATCCTAA CTCTTTCCCT TCTAACCCCT TCCTTGGCCC 6961 TTCTTCCTGC CCGGGAGGAT CAAGGGCGCT GTTTTCCAGA AGCTGCCGGG TCGGAAACGG 7021 TGGGGGAACG GGGCGGTACG CGTGGGTGGA GGATAGGCGG GGAGGGGGCA GTCCACGAAC 7081 TTTTGTCATT CAGCCTGGGA GACCACGGTG ggatccCACC ATGGAAGATG CCAAAAACAT 7141 TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA 7201 CAAAGCCATG AAGCGCTACG CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT 7261 CGAGGTGGAC ATTACCTACG CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT 7321 GAAGCGCTAT GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA 7381 GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA 7441 CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT 7501 CGTGAGCAAG AAAGGGCTGC AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA 7561 AAAGATCATC ATCATGGATA GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT 7621 CGTGACTTCC CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA 7681 CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG 7741 CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT 7801 CGGCAACCAG ATCATCCCCG ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT 7861 CGGCATGTTC ACCACGCTGG GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG 7921 CTTCGAGGAG GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT 7981 GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG 8041 CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT 8101 GGCCAAACGC TTCCACCTAC CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG 8161 CGCCATTCTG ATCACCCCCG AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC 8221 CTTCTTCGAG GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG 8281 CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC 8341 TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA 8401 CGAGGACGAG CACTTCTTCA TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA 8461 CCAGGTAGCC CCAGCCGAAC TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC 8521 CGGGGTCGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT 8581 GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC 8641 AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC 8701 CGGCAAGTTG GACGCCCGCA AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA 8761 GATCGCCGTG TAATTCTAGT TGTTTAAACG AGCTCGCTAG CCTCGAGTCT AGAGTCGACC 8821 TGCAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.