pLV2-CMV-Shox2-GST-Puro plasmi

pLV2-CMV-Shox2-GST-Puro plasmid

PVT110406

pLV2-CMV-Shox2-GST-Puro plasmid Information

Plasmid name: pLV2-CMV-Shox2(mouse)-GST-Puro
Alias: NM_013665.1;6330543G17Rik; OG12; Og12x; Prx3; SHOT
Gene lenght: 993
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Shox2-GST-Puro plasmid Description

pLV2-CMV-Shox2-GST-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-CMV-Shox2-GST-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 GCCACCatgg aagaacttac ggcgttcgtc tccaagtctt ttgaccagaa agtgaaggag 2881 aagaaggagg ccatcacgta ccgggaggtg ctagagagcg ggccgctgcg cggggccaaa 2941 gagcccggtt gcgtcgagcc gggccgcgac gaccgcagca gcccggcagt ccgggcggcc 3001 ggcggaggcg gcggcgcggg aggaggcgga ggcggaggcg gaggaggcgg aggaggtgct 3061 ggaggaggag gagcaggcgg aggagctgga ggagggcgct ctcccgtccg ggagctggac 3121 atgggagccg cggagcggag cagggagccc ggcagcccgc ggctgacgga ggtgtcccct 3181 gaactgaagg atcgcaaaga cgatgcgaaa gggatggagg acgaaggcca gaccaaaatc 3241 aagcagaggc gaagtcggac caattttacc ctggaacaac tcaacgagct ggagaggctt 3301 ttcgatgaga cccactatcc agacgctttc atgcgcgagg aattgagcca gcgactgggg 3361 ctctctgagg cccgagtaca ggtttggttt caaaatcgaa gagctaagtg tagaaaacag 3421 gaaaatcaac ttcacaaagg tgtccttata ggagccgcta gccagtttga agcttgtaga 3481 gttgcaccct atgtcaacgt aggtgcttta aggatgccat ttcagcagga tagtcattgc 3541 aacgtgacgc ccttgtcctt tcaggttcag gcgcagctgc agctggacag cgccgtggcg 3601 cacgcgcacc accacctgca tccgcacctg gccgcgcacg cgccttacat gatgttcccg 3661 gcaccgccct tcggactgcc gctggccacg ctggccgcgg actcggcctc ggccgcctcg 3721 gtggtggccg ctgccgccgc cgccaagacc accagcaaga actccagcat cgcggatctc 3781 agactgaaag ctaaaaagca cgcggccgcc ctgggtctgg gtggaggcgg atcagaattc 3841 gccaccatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga 3901 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt 3961 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat 4021 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag 4081 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg 4141 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc 4201 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt 4261 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct 4321 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt 4381 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat 4441 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa 4501 tgaggatccc gcgactctag ataattctac cgggtagggg aggcgctttt cccaaggcag 4561 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg 4621 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc 4681 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg 4741 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc 4801 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc 4861 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg 4921 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa 4981 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc 5041 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 5101 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 5161 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 5221 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 5281 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 5341 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 5401 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 5461 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 5521 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 5581 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 5641 ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg 5701 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 5761 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5821 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 5881 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 5941 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 6001 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 6061 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 6121 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 6181 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 6241 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca 6301 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 6361 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 6421 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc 6481 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 6541 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca 6601 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct 6661 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga 6721 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca 6781 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc 6841 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt 6901 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 6961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7081 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7141 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag 7201 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 7261 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7321 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7381 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7441 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8041 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 8101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8281 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8341 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8401 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8461 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8521 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8581 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8641 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8701 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8761 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8821 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8881 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8941 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9001 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9061 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9121 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9181 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9241 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9301 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9361 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg 9421 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 9481 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa 9541 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt 9601 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa 9661 attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta 9721 aatatgtact acaaacttag tagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.