pMFSD2A-Fluc-SV40-hRluc-Neo pl

pMFSD2A-Fluc-SV40-hRluc-Neo plasmid

PVT110396

pMFSD2A-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pMFSD2A(human)-Fluc-SV40-hRluc-Neo
Alias:
Gene lenght: 2101
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc,Rluc
Promoter: MFSD2A
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: cer region-F1
Reverse primer:
Copy number: High
Induction:

pMFSD2A-Fluc-SV40-hRluc-Neo plasmid Description

pMFSD2A-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pMFSD2A-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattcC TTTGTCTGTC ACCCAGGCTG GAGTGCAGTG GCACGATCTC GGCTCACTGC 5161 AACCTCCGCC TCCTGGGTTC AAGCGATTCT TCTGCCTCAG CCTCCTGAGT ACCTGGGACT 5221 ACAGGTGTGC CCCACCACAC CCGGCTTATT TTTGTATTTT TAGTTGAGAC AGGGTTTCAC 5281 CATATTGGCC AGGCTGGTCC GGAACTCCTG ACTTCGTGAT CCACCCACCT CAGCCTCCCA 5341 AAGTGCTGGG ATTACAGGCG TGAGCCAGGG TGCCTGGCCA GTAGCTACTA TTAATATCAC 5401 CATTTTACAC AGGGAGGTTA ACTGAGTTGT TCAGAGTCAG CCGCACAGCT AGTAAATGGA 5461 GACACTTAGG GCTTTTCCTA ATGGGTTTCT GTGTGCTTGT ACCTTAGCAG GGGAAGACTG 5521 AACATTCATT CACTGAACAC AATAATTCAG GCCCTAAGCT AGTTGGTGGG GACACAGAAG 5581 TGAAAAAGAT GCGGTTCCTA CCTGAGGAGC TCATTGTCAA CCGGGGGATA CAGATCACTT 5641 GTGGTATGGT GTGACTGTGG GAGAGAAGAT TTGGGGGCTG CAAGGGAGCT TAGCTAACCA 5701 AGCCTGGAGA GGGGAAAAGA GACAGAACAG TGTCACACAG CTTGGAGTAA TGGGGCCAGC 5761 CATTCCCTTT CCACAGTATT CAGTGCCTCA CTTTGGCTCT CTTCTGCTCA GCAATGGCCA 5821 AGAGCTGTGT GCCCAGATGG GATCTGCTGG CGTATGAGGG AGCATGGTGT GACCATCCTG 5881 GGGCAGATAG ACCTTATCTC TCCTCCCTGC AATCAGAGAT TTAAAGAAAG GAAGAAGGAG 5941 GCTGACATTA AGGAGCACAG GAACCGGGTG GGTAGCCTTT GAGAGTTCCA GCAGCCCCTT 6001 CACTAATCAG AGAACTGGAA AGACCGGGGT GACATTGACA TAAGGGACTC AGAGGTGGAA 6061 CTAAAGACTG TTCTTTTCTG GCATCCGTGC CTGCCTTCTG TTCCAAACTA ATGCCCTCCC 6121 ATCTTCTTTT GAGGACCACA TTTCTGGGCA TTCTCTTTTT CAAAACTTTC CCTGTTTCAA 6181 CCCTCAGTGC CTCTGGAGCC ATGTATCTCC CAGGGCTTCT TGTTGGGAGA CACATACACC 6241 CCATTCTGCC GTCTGTAATT TATGCTAAGA GGGATAGAGC AGGGCTGTTG AGAGCAACTG 6301 CTTTGGTATC GGACAAAACC TTTCAGGCCT GGCTCTGCTA CATAACAGTT GCTTCACTTC 6361 TCCAAGTTTC CCCTTCCTGT GCTGTAAAAT GGGGATAATA ATAGTACCAC CCTCACTTGG 6421 TGGTTTTTGA GGGCCGAAAG AGATCATATT AAGCATGGAG GAAGCTATCC ATAAAAAGGC 6481 CACTGTTGTC ATAGTGTACT AGCGGGCGTA GCCCCGGCTT CTCTCCACGA CTTGGGAGCT 6541 CCCTTCTGGC AGGGGCAGCC ACTGGATTGC CTCATCTTTC TCCCCATTGC CCGGATGCCA 6601 AGGGCAGGAC GGGGGAATGG TGTGTCTTCG GGGATGAGAG AGAGGCATCA GGAGGGAAAG 6661 TGTCAGCGTA CGTCCTCTAG GCTTGAAGAA GGAGAGAGGC CCTGACTCCA GATTTTAAAG 6721 TGGGCTTCAG AGCCCCTACC TTTCCTAGGC CTGGGGCTCA GAAGTCATGG GTGGTAGGCC 6781 CGACTCGGGG AGAGTCGGAC CTTGGGGACC TCGGGGACCT CGGGCAGATC GGGGACCCCG 6841 GGCAGATCAC TTTCAGCCTG GGGCCTCGGT TTCCTCGCGC GTGTCTGGGA GGTCTCGCGG 6901 CCTGCTTCTC GGATTGCTAG GAGCCAGCGC AGGCAGATGC CGGTTCCGGG GCTTGGAGAA 6961 CGTGGCTCGG TGGCACAGAG CCGCCGGGCG AGGCGGGGGC AGGGAGGCGG GCGACGGGAT 7021 TGGCTGGAGG CGGGGCAGCG GGGCTCGGGG ATTGGCCGCA ACCGAGAGGG CTGGGCAGAA 7081 CTATAAGAGG CGCGGAGGGG GCGTGCAGCA GAGTGCGTTC CTCGTCTGCC AGCCGGCTTG 7141 GCTAGCGCGC GGCGGCCGTG GCTAAGGCTG CTACGAAGCG AGCTTGGGAG GAGCAGCGGC 7201 CTGCGGGGCA ggatccCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT 7261 TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG 7321 CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG 7381 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA 7441 CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG 7501 GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC 7561 TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC 7621 AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA 7681 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC 7741 CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC 7801 TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA 7861 CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG 7921 ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG 7981 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT 8041 TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT 8101 TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA 8161 GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC 8221 CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG 8281 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG 8341 TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG 8401 GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA 8461 AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA 8521 TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC 8581 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG 8641 ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA 8701 CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG 8761 GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA 8821 AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATTCTAGT 8881 TGTTTAAACG AGCTCGCTAG CCTCGAGTCT AGAGTCGACC TGCAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.