pCMV-Zbtb40-3×FLAG-Neo plasmid

pCMV-Zbtb40-3×FLAG-Neo plasmid

PVT110374

pCMV-Zbtb40-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Zbtb40(mouse)-3×FLAG-Neo
Alias: NM_198248.1;C230087D24; Gm571; mKIAA0478
Gene lenght: 3774
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Zbtb40-3×FLAG-Neo plasmid Description

pCMV-Zbtb40-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Zbtb40-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgga actccccagc tacagccagc 781 agctgcttct gcagctctac gccctgtgca aggagcagcg gttctgtgat tgcaccattt 841 ccgtcggcaa catctacttc agggcccaca agccagtcct ggcagcagcc agcctcctgt 901 tcaaaaccct gctggacagc acagacgcca tctccatcga tgcatccgtg gtgagccccg 961 aagagtttgc cctcttgcta gaaatgacat acaccggtaa gctacctgtg ggcaagcaca 1021 acttctccaa gatcatctcc ttagcagata gcctgcagat gtttgacgtg gccgttagtt 1081 gtaaaaacct tctgaccaac ctggtaaact gctctgtcca ggggcaggtg gtaagagatg 1141 tctcagtgct gtcctcagag gcagggaaca aggagtcaga gaagccacaa gtagaagccc 1201 tctcctctga aggtgctgag gagccctctt cttccccgga agtttctgcc gtgccagcgg 1261 gccctgagaa agccaagacc caggacgtaa tcaacatagc tacccaagag ataaatgcag 1321 accctcccgt tgcccaagag gttcaggtgg ctgcagagat cacaggggcg actcctcaca 1381 cagctgaagt ttgttcccct tcccctgttg gacaaatccc aagtgagacg aaagaagtca 1441 atacagaagc agagtgcgag acaaagcagg accaactgac tctcctgcta gagcatgaaa 1501 gcgtcttctc agatgcagcc ttgctcaccc cagacaccct gagaagactg gcaggatgct 1561 cagagattga agggcctcag aaggagacca taatagaatg tctcacgagt gaaggaggca 1621 gttcagcgtt ccagagaatt ctggacaaag tgcacgatgg gagcctggat gtgcaggtgg 1681 ctctgtctct ggtgagacta taccaggagt ctacacctgc agagaaagtg tctcagattc 1741 aaccagaagg gagcgcagga gagggaaaga ccttgtctgt tctgctactg gaacacaaag 1801 aggacctgat ccagtgtgta acacagctca gacctattgt ggagttcctg gaaacagcca 1861 aggaggaatt cctgccagat tccgaaaaaa gggtgattca gagctgctgt gagggcagaa 1921 cacccaagga gatgatagag aatttgttgc acagagtgac tgaagagaag accctgcctg 1981 ccaagagtct ggtaaaactc ctccaggctg tgaggacagc attcccaaac ctggacctcc 2041 tgctggacaa tttgcagaaa ggagcgggat cagccggcac cacaggcctg gccagagtct 2101 cctgtgaatc agaatggaca gatgagccca ggccagtgtg cattccagca gaccgcgtgg 2161 atggatcctc ccctagccct gatgcttacg gagctgagct gttgagaaag taccatgaga 2221 acctgtctga gattctcata gacaaccagg tgctgctgaa ggtgatctca cggatgcaaa 2281 gcttagcccc tggagacaga gaggtcatgc agaccctcgt gaaacagggc tctggctcgg 2341 gtggcttcag ttccctgatg ttggcagctc tggaaaggca gactctctca gcaacggcca 2401 tttggcaact actgctagca gttgaagaga ctgagacccg cccactgaac ctgctcttgg 2461 aggaagtagg aaaggagccc ggcgctgggg ctttcttccg ggcagtgact gccccagaga 2521 gcacagtatt ggaagccatc ctgaggcaca gcaagctgct ccgggaagcc atccagcaga 2581 cagcggggtg tgagcgcctt tcttcagagg aagaacacct ggcagagacc gtgcaagaga 2641 tgctgagcat atcctccgag acagtgagcc ctgaagcctc cctgagagcg gtgctgagca 2701 aagccatgga gaaatctgtc tcggcctctg tggtctgtca cctcctctgc agtgtccaca 2761 agtctttccc tggcctgcag cccctgatgc aggagttggc acatgttggt ttccttacca 2821 aggaaagcgg agaggagagg tggaaggtga ctgatggcct tcaccctgga gccaaggaca 2881 aagtggagga agtggccaag gcaaccggag aagactgcca ggccaatgaa caaaaggagc 2941 agggagagcc tgggcccgtg ccagagcaac cggagaaaga cacttctgcc tccccagact 3001 ctgccaagaa gagcttcgtc tgcaaagcct gtgataagag cttccatttc tactgccgtc 3061 tgaaggtgca catgaagcgc tgccgggtgg ccaagagcag gcaggtgcag agcgaggagg 3121 gcagcgagac caagggctcg gagaaggggc tggagaagca gcagccggac gcccgcggca 3181 caggtgggga gcccgatgcc cccaagaaga agaagaagcg gctgccagtg acatgtgacc 3241 tctgcggaag agagtttgcc catgcctcag gcatgcagta ccacaagctg accgagcact 3301 tcgacgagaa acctttctcc tgtgaggaat gtggggcgaa gtttgcagcc aactctaccc 3361 tgaagaacca cctgcgcctg cacaccggag accgcccgtt catgtgcaag cattgcctca 3421 tgaccttcac gcaggcctcc gccctggctt accacaccaa gaagaagcac tcggaaggga 3481 aaatgtacgc gtgccagtac tgtgacgccg tgttcgccca gtcgatcgag ctatcccggc 3541 atgtgaggac ccacaccggg gacaagccat atgtctgtag ggactgtgga aagggcttcc 3601 ggcaagccaa cggcctctcc atccacctgc acaccttcca cacagacata gaagaccctt 3661 atgactgcaa gaaatgcagg atgagcttct ccacactgca ggaccaccgg aagcacatcc 3721 acgaggtgca cgccaaggag taccacccct gccccacgtg cggcaagatc ttcagtgccc 3781 cttccatgct ggagcggcac atggtgaccc acgtgggagg gaagcccttc agctgtggga 3841 tctgcaacaa ggcctaccag caattgtctg gattgtggta ccacaatcgg acgcaccacc 3901 cagatgtgtt tgctgctcag aaccatcggt cacccaagtt ctcatcactg cagtgtagct 3961 cctgtgacaa gaccttttcc aatactgtcg aacacaagaa gcacatcaaa acagaacaca 4021 cagatgtgaa gttccatgag tgtgaccagt gcaaggagct cttccccaca cctgctctgc 4081 tccaggttca cgtcaagtgc cagcattcag ggtcgcagcc attccggtgc ttgtactgtg 4141 cagccacctt ccgcttcccg ggagccctgc agcaccacgt caccacagag cactttaagc 4201 agtcggaaag caccttcccc tgcgagctct gtggcgagct cttcacctcc caggcccagc 4261 ttgatagcca cttggagtct gagcacccaa aggtggcagg cactgagtcc caggcagctg 4321 cctctcgcat ggtgcaggtg atccgagctc cggagccagc ggccccggcc gagcaggtga 4381 tcacgttgga ggagacgcag ctcgctggct cgcaggtgtt tgtgaccttg ccagattcac 4441 agacctctca gaacagctcc gagcttgtgg cagtgacggt ggaggatttg ctggatggta 4501 ctgtcacatt gatctgtggt gaggccaagG GATCCGACTA CAAAGACCAT GACGGTGATT 4561 ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGAGGG CCCGATATCT 4621 CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT 4681 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC 4741 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA 4801 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC 4861 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT 4921 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG 4981 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT 5041 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC 5101 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG 5161 TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA 5221 CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG 5281 GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT 5341 TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC 5401 CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC 5461 AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG 5521 GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 5581 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 5641 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT 5701 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 5761 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 5821 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 5881 TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 5941 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 6001 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 6061 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 6121 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 6181 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 6241 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 6301 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 6361 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 6421 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 6481 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 6541 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 6601 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 6661 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 6721 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 6781 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 6841 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 6901 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 6961 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 7021 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 7081 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 7141 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC 7201 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 7261 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 7321 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 7381 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 7441 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA 7501 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 7561 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 7621 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA 7681 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC 7741 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 7801 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 7861 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 7921 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 7981 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 8041 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 8101 TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 8161 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 8221 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 8281 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 8341 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 8401 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 8461 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 8521 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 8581 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 8641 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 8701 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 8761 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 8821 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 8881 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 8941 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC 9001 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 9061 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 9121 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 9181 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 9241 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 9301 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 9361 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 9421 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 9481 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT 9541 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG 9601 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA 9661 CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT 9721 GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG 9781 TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT 9841 CTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.