pLV3-CMV-Osbp2-3×FLAG-3UTR-Hyg

pLV3-CMV-Osbp2-3×FLAG-3UTR-Hyg plasmid

PVT110334

pLV3-CMV-Osbp2-3×FLAG-3UTR-Hyg plasmid Information

Plasmid name: pLV3-CMV-Osbp2(rat)-3×FLAG-3UTR-Hyg
Alias:
Gene lenght: 1623
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Osbp2-3×FLAG-3UTR-Hyg plasmid Description

pLV3-CMV-Osbp2-3×FLAG-3UTR-Hyg It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-Osbp2-3×FLAG-3UTR-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCAGGA TTAGGATTAG GAAGAGCAAA AGCGATTAGA GCTCGTCCTT 2341 CTTCTGGGTA CAGCGAACAG CGGTTTCCAG GTCAGAAACT GTGTATCCCA TGGACACCTG 2401 CTAAGAAGGG AGCTCAGCAC TCCAGGGCAG CAGAGCCAGG CCGGCTCCTG TCTCTTCACG 2461 GCGTGGCCAG CATGAGGCCT GGAGAAGGCC AGTGGCAGCT GAGAAGACCC TGGGAGCTGG 2521 GGGTGGAAAG CAGGCCTGTA AGGACTTCCT GGAACTAGCG GAGATTCACA GCCGGAAATG 2581 GCAGCGGGCT CTGAATTATG AGCAGGAGCA ACGAGTCCAC CTGGAGGAAA CCATTGAGCA 2641 GTTGGCCAAG CAGCACAACA GTCTTGAACG GGCCTTCTGC AACACCCCTG GCGGGCCAGC 2701 CAGCTCCAGC AAGAGCTTCA GCGAGGGAAG CTTCTTGACT TCCAAAGGGG AGAACAGTGA 2761 AGAAGATGAA GATACTGAGT ACTTTGATGC CATGGAAGAC TCCACATCTT TCATCACCGT 2821 AATCACCGAG GCCAAGGAAG ACAGAAAGCC TGAGGGTGGA CCTGGGACGA CCTCTGTGGA 2881 CTGGACCTCA GCAGATAATG TATTAGATGG TGCCTCATTC ATGCCCAAGG ATTCATGCAA 2941 AGTGAAGAGG CGAGTCCGGA TCCCTGACAA ACCCAACTAT AGCCTTAACC TCTGGAGCAT 3001 CATGAAGAAT TGCATTGGCC GAGAGCTCTC CCGGATCCCC ATGCCGGTGA ACTTCAATGA 3061 GCCCCTGTCC ATGCTCCAGC GACTTACAGA GGACCTCGAG TACCACCACC TGCTGGACAA 3121 GGCGGTGAAC TGCACCAGCT CTGTGGAGCA GATGTGCCTG GTGGCTGCCT TCTCGGTGTC 3181 CTCCTACTCC ACCACCGTGC ACCGCATCGC CAAGCCCTTC AACCCGATGC TGGGGGAGAC 3241 CTTCGAGTTG GATCGCCTGG AGGACATGGG CCTGCGTTCT CTCTGCGAGC AGGTGAGCCA 3301 TCACCCCCCG TCTGCTGCCC ACCACGTGTT CTCTAAGCAT GGCTGGAGCC TCTGGCAGGA 3361 GATCACCATC GCCAGCAAGT TCCGGGGGAA ATACATCTCC ATCATGCCAC TTGGTGCCAT 3421 CCACCTCGAA TTCCAGGCCA GTGGCAATCA CTACGTGTGG AGGAAGAGTA CCTCCACTGT 3481 GCACAACATC ATCGTGGGCA AGCTCTGGAT TGACCAGTCA GGGGACATTG AGATTGTGAA 3541 CCACAAGACC AAGGACCGGT GCCAGCTGAA GTTCGTGCCC TACAGCTACT TCTCCAAAGA 3601 GGCAGCCCGA AAGGTGACTG GAGTGGTGAG CGACAGCCAG GGCAAGGCCC ACTACGTGCT 3661 GTCAGGTTCC TGGGATGAGC AGATGGAGTG TTCTAAGATT GTGCACAGCA GCCCCAGCTC 3721 TGATGGGAAG CAGAAAACCG TGTACCAGAC GCTGCCAGCC AAGCTGCTAT GGAGGAAATA 3781 CCCACTGCCG GAGAACGCGG AGAACATGTA CTATTTCTCG GAGCTGGCCC TGACCCTCAA 3841 CGAGCAGGAG GAGGGCGTGG CGCCCACCGA CAGCCGCCTG CGGCCGGACC AGCGGCTGAT 3901 GGAGAGGGGC CGCTGGGACG AGGCCAACAC CGAGAAGCAG CGCCTGGAGG AGAAGCAGCG 3961 CCTGAACCGG CGACGGCGGC TGGAGTCGTG CACGGCCGGC TGTGGCGGGG AGGAAGAGAA 4021 GGAGTCAGAT GGCTATTCGC CGCTGTGGTT CGAGAAGAGG CTGGACCCGC TGACCGGGGA 4081 GATGGCCTGC ATATACAAGG GCGGCTACTG GGAGGCCAAG GAGAAGAAGG ACTGGCACAT 4141 GTGCCCCAAC ATCTTCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA 4201 CTACAAGGAT GACGATGACA AGTAGGCCAC TCCTGCGGAA AATACAGGCA CCTGCACTGC 4261 CTGTCAGACT TTTCTTTAAT GCACCCGATT TAATACAGAA CCCACCAATG CGTGTGTGCG 4321 CACGCGCGTG ACCACACCGC TTAAGAAGGG GTAAGGGCTG ACCTGGGTTC TTGCCAGCCC 4381 CAGGTCGCCG GGTCACGTGT GCCCGTTGTT ATCATTGACT TGGGCCCCAT GGGCACCCTA 4441 GTTCCCAGTT ACCACAACTC AGGCCGGCTG GCCGGGCCAT GGGCTGCCCA GGTCACTAAC 4501 CCCAACCCAG GGAGGAAATA TTTCCCTCCT AGGGAGCCTC TTTTGACTTT TTTAAGAAAA 4561 AAAAATATCC ATTTCTTTCC AACGATGAGG TTTAGTAAAT ATTTTTAGCA CATCACTTAG 4621 AAGACTCACT TTCCAAGTGT TCGTTCTTGC CACAGAACGG TTCTGGCAAG AAGCCCGTTT 4681 TTAAAGACCT GAGGAAGCCA GGCCCCTTTG AATGGGGAAC ACTTCAAACA CAGCCCGGCT 4741 TTCTAGCTGG ACTGTCCAAG CTCCAGGAGC GCAACACCCG CAGAGACAGC AGGAAGCCTG 4801 GCTGGCCGGC CCAGCATGGC ATGCAGAGCC CGAAGGGGGA AGGCAGTCAA AGGCAGCCGC 4861 CCCCCCCCCC CCCGTGCCAG GCCTTGGCCA TGGTGGGGCA GTATCCCAGT CCCAAAGAGG 4921 CCAGGAAGGG CTGAAAGGCA GGGCCCTTAG GTGCTTCCTG CCCAAGAATC AGGCCCCCAA 4981 ATCAGCAATA AGGAACAAAC CCTTCCTTTA GCCTGGGCTG GTGACCCAGG CTCCCAACAC 5041 CTGGCCTCCC GCCTTGTCCT TCGGCGGCAT CAGGGTGCCT CACTCCGAAG TCCTGACATC 5101 TTTATTTGCC TAATTTATAT ATATATATGA TATACAAATA TATATGACAT AGCTATTTTG 5161 CTTAAATTTC TATGATACGT GAAAGTGAAA AATGATGAAG ACAGACTGCC TGTCTGAGTT 5221 TGGCCTCCTG CCAGCCCCGC CCTCTCCTTG TAGCCCTCCC AGTGGCTTCT GCCAACCTTA 5281 GAAATGAAAC CTGCCCCTCA GACCCTCTGC CTCCAACCCA TCTCACTTCA GTTTGATCTG 5341 GAAAGTTCAT TGCAGGGCCT TTCTTTCTAC TCCAGGCCTG GTTTTGTTTT TTGTTTTGTT 5401 TTTTATTAAA AAAGAAAAAG AAAACACCAC GTATGAAATG CCTTACAATG CCCACTCTAG 5461 AGAGGGGGTG GGAAGGGCGG GGGAGGGAAG GTCACTCGGT CTCCCTCAGA GCTGGAATGT 5521 ACCCCTTCCC AGCAGGCTGA GCAAGCAAGC ACCTTCTGAT CATAATAAAA ACCTTGGTTC 5581 TGGAGTTTTC CACAGCCCGc ggccgcaagg atctgcgatc gctccggtgc ccgtcagtgg 5641 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 5701 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 5761 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 5821 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 5881 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 5941 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 6001 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 6061 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 6121 cgttacagat ccaagctgtg accggcgcct acgtcgacat gaaaaagcct gaactcaccg 6181 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc 6241 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc 6301 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg 6361 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga 6421 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac 6481 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta 6541 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc 6601 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg 6661 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact 6721 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca 6781 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg 6841 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct 6901 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc 6961 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt 7021 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac 7081 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata 7141 gtggaaaccg acgccccagc actcgtccga gggcaaagga ataggtcgac aatcaacctc 7201 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 7261 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 7321 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 7381 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 7441 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 7501 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 7561 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg 7621 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 7681 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 7741 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg 7801 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 7861 ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta 7921 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 7981 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 8041 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 8101 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac 8161 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 8221 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 8281 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 8341 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 8401 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt 8461 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 8521 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 8581 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 8641 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 8701 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 8761 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 8821 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 8881 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 8941 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 9001 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 9061 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 9121 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 9181 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 9241 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 9301 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 9361 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 9421 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 9481 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 9541 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 9601 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 9661 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 9721 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 9781 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 9841 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 9901 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 9961 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 10021 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 10081 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 10141 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 10201 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 10261 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 10321 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 10381 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 10441 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 10501 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 10561 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 10621 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 10681 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 10741 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 10801 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 10861 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 10921 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 10981 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 11041 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.