pLV3-EF1a-MAEA-6×His-BSD plasm

pLV3-EF1a-MAEA-6×His-BSD plasmid

PVT110325

pLV3-EF1a-MAEA-6×His-BSD plasmid Information

Plasmid name: pLV3-EF1a-MAEA(human)-6×His-BSD
Alias: NM_001017405.3;EMLP; EMP; GID9; HLC-10; P44EMLP; PIG5
Gene lenght: 1188
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: 5' LTR (truncated) -F1
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-MAEA-6×His-BSD plasmid Description

pLV3-EF1a-MAEA-6×His-BSD It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-EF1a-MAEA-6×His-BSD plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gcggtgcagg agtcggcggc 2521 tcagttgtcc atgaccctga aggtccagga gtacccgacc ctcaaggtgc cctacgagac 2581 gctgaacaaa cgctttcgcg ccgctcagaa gaacattgac cgggagacca gccacgtcac 2641 catggtggtg gccgagctgg agaagacgtt gagcggctgc cccgccgtgg actccgtggt 2701 cagcctgctg gacggcgtgg tggagaagct cagcgtcctc aagaggaagg cggtggaatc 2761 catccaggcc gaggacgaga gcgccaagct gtgcaagcgc cggatcgagc acctcaaaga 2821 gcatagcagc gaccagcccg cggcggccag cgtgtggaag aggaagcgca tggatcgcat 2881 gatggtggag cacctgctgc gttgcggcta ctacaacacg gctgtcaagc tggcgcgcca 2941 gagcggcatc gaggacctag tgaatattga gatgttcctg acggccaaag aggtggagga 3001 gtccctggag aggcgtgaga cggccacctg cctggcctgg tgccatgaca acaagtcccg 3061 gctccggaag atgaagagct gcctggagtt cagcctcaga atccaggagt tcattgaact 3121 catccggcag aataagagac tggacgctgt gagacatgca agaaagcact tcagccaagc 3181 agaagggagc cagctggacg aggtgcgcca ggccatgggc atgctggcct tcccgcccga 3241 cacgcacatc tccccgtaca aggaccttct ggaccctgca cggtggcgga tgctgatcca 3301 gcagttccgg tacgacaact accgactaca ccagctggga aacaattctg tgttcaccct 3361 caccctgcag gctggcctct cagccatcaa gacaccacag tgctacaagg aggacggcag 3421 ctccaagagc cctgactgcc ctgtgtgcag ccgctccctg aacaagctgg cgcagcccct 3481 gcccatggcc cactgtgcca actcccgcct ggtctgcaag atttctggcg acgtgatgaa 3541 cgagaacaat ccgcccatga tgctgcccaa cggctacgtc tacggctaca attctctgct 3601 ttctatccgt caagatgata aagtcgtgtg cccgagaacc aaagaagtct tccacttctc 3661 acaagccgag aaggtgtaca tcatgCATCA TCACCATCAC CATTGAgcgg ccgcTAGtgg 3721 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc 3781 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc 3841 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg 3901 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt 3961 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg 4021 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt 4081 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc 4141 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc 4201 tcatctccgg gcctttcgac ctgcagccca agcttaccAT GGCCAAGCCT TTGTCTCAAG 4261 AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC ATCTCTGAAG 4321 ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT GGTGTCAATG 4381 TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT GCTGGGCACT GCTGCTGCTG 4441 CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG GGCATCTTGA 4501 GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA TCCTGGGATC AAAGCCATAG 4561 TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG CCCTCTGGTT 4621 ATGTGTGGGA GGGCTAAgtc gacaatcaac ctctggatta caaaatttgt gaaagattga 4681 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 4741 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 4801 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4861 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 4921 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 4981 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 5041 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 5101 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 5161 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 5221 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 5281 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 5341 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 5401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 5461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 5521 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 5581 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 5641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 5701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 5881 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 5941 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6001 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6061 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6121 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6481 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6541 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6601 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6661 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6721 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 6781 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6841 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6901 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6961 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7021 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7081 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7141 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7201 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7261 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7321 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7381 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7441 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7501 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7561 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7621 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 7681 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 7741 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 7801 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 7861 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 7921 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 7981 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8041 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 8101 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 8161 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 8221 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 8281 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 8341 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 8401 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 8461 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 8521 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.