pLV3-CMV-PPDPF-3×FLAG-CopGFP-P

pLV3-CMV-PPDPF-3×FLAG-CopGFP-Puro plasmid

PVT110319

pLV3-CMV-PPDPF-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PPDPF(human)-3×FLAG-CopGFP-Puro
Alias: NM_024299.4;C20orf149; dJ697K14.9; exdpf
Gene lenght: 342
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PPDPF-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PPDPF-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-PPDPF-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cggccatccc ctccagcggc 2341 tcgctcgtgg ccacccacga ctactaccgg cgccgcctgg gttccacttc cagcaacagc 2401 tcctgcagca gtaccgagtg ccccggggaa gccattcccc accccccagg tctccccaag 2461 gctgacccgg gtcattggtg ggccagcttc tttttcggga agtccaccct cccgttcatg 2521 gccacggtgt tggagtccgc agagcactcg gaacctcccc aggcctccag cagcatgacc 2581 gcctgtggcc tggctcggga cgccccgagg aagcagcccg gcggtcagtc cagcacagcc 2641 agcgctgggc ccccgtccgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 2701 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 2761 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 2821 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 2881 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 2941 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3001 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3061 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3121 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3181 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3241 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 3301 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3361 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 3421 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3481 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3541 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3601 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 3661 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 3721 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 3781 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 3841 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 3901 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 3961 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4021 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4081 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 4141 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 4201 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 4261 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 4321 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 4381 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 4441 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 4501 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 4561 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 4621 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 4681 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 4741 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 4801 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 4861 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 4921 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 4981 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5041 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5101 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 5161 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 5221 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 5281 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 5341 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 5401 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 5461 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 5521 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 5581 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 5641 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 5701 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 5761 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 5821 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 5881 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 5941 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 6001 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6061 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6121 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6181 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6241 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6301 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6361 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6421 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6481 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6541 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6601 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6661 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6721 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6781 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6841 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6901 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 6961 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7021 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7081 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7141 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7201 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7261 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7321 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7381 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7441 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7501 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7561 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7621 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7681 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7741 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7801 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7861 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7921 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 7981 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8041 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8101 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8161 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8221 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8281 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8341 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8401 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8461 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8521 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8581 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.