pLV3-CMV-PACSIN1-3×FLAG-CopGFP

pLV3-CMV-PACSIN1-3×FLAG-CopGFP-Puro plasmid

PVT110095

pLV3-CMV-PACSIN1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PACSIN1(human)-3×FLAG-CopGFP-Puro
Alias: NM_020804.5;SDPI
Gene lenght: 1332
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PACSIN1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PACSIN1-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-PACSIN1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgt ccagctccta cgatgaggcc 2341 tcactggcgc cagaggagac caccgacagc ttctgggagg tggggaacta caagcggacc 2401 gtgaagcgca tcgatgacgg ccaccgtcta tgcaacgacc tgatgaactg cgtgcaggag 2461 cgcgccaaga tcgagaaggc gtacgggcag cagctcaccg actgggccaa gcgttggcgc 2521 cagctcatcg agaaaggccc acagtatggc agcctggagc gggcctgggg tgccataatg 2581 acagaggcag acaaggtgag cgagctgcac caggaggtga agaacaatct gctgaatgag 2641 gacctggaga aggtgaagaa ctggcagaag gacgcctatc acaagcagat catgggtggc 2701 ttcaaggaga cgaaggaggc tgaagatggc ttccgcaagg cccagaagcc ttgggccaag 2761 aagatgaagg agctggaggc agccaagaag gcctaccatt tggcttgcaa agaggaaaag 2821 ctggccatga cacgggagat gaacagcaag acggagcaat cggtcacacc tgagcagcaa 2881 aagaagctgc aggacaaagt ggacaagtgc aagcaggatg tgcagaagac acaggagaag 2941 tatgagaaag tgctggaaga tgtgggcaag accacacccc agtacatgga gaacatggag 3001 caggtgtttg agcaatgcca gcaatttgag gaaaagcggc tggtcttcct caaggaggtg 3061 ctgctggaca tcaaacggca cctcaacctg gctgagaaca gcagctacat ccatgtgtac 3121 cgtgagctgg agcaggccat ccggggggct gatgcccagg aagacctcag atggttccgc 3181 agcaccagtg gccccggcat gcccatgaac tggccccagt ttgaggagtg gaacccagac 3241 cttcctcaca ccaccaccaa gaaggagaaa cagcctaaga aggcagaggg agtggcgctg 3301 accaatgcca ctggggcggt agagtccaca tcccaggctg gggaccgcgg cagtgttagc 3361 agctacgaca gaggccagcc ctacgccacc gagtggtcag acgacgagag tgggaacccc 3421 tttgggggca gtgagaccaa cgggggcgcc aacccctttg aggacgactc caagggagtg 3481 cgcgtgcggg cactctacga ctatgacggc caggagcagg acgagctcag ctttaaggcc 3541 ggagacgaac tcaccaagct gggcgaggag gatgagcagg gctggtgccg tgggcggctg 3601 gacagcgggc agctgggcct ctaccctgcc aactacgtgg aggctatcgg atccGACTAC 3661 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 3721 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3781 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3841 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3901 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 3961 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4021 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4081 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4141 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4201 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4261 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4321 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4381 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4441 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4501 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4561 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4621 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4681 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4741 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4801 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 4861 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 4921 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 4981 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5041 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5101 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5161 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5221 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5281 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5341 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5401 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5461 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5521 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5581 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5641 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5701 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5761 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5821 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5881 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 5941 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6001 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6061 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 6121 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6181 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 6241 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 6301 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 6361 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 6421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 6481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 6541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 6601 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 6661 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 6721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 6781 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 6841 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 6901 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 6961 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 7021 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7081 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7141 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7201 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 7861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8041 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8101 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8161 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8221 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8281 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8341 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8401 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8461 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8521 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8581 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8641 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8701 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8761 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8821 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8881 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8941 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9001 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9061 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9121 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9181 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 9361 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 9421 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 9481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9601 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.