pLV3-CMV-PKN3-3×HA-Puro plasmi

pLV3-CMV-PKN3-3×HA-Puro plasmid

PVT110092

pLV3-CMV-PKN3-3×HA-Puro plasmid Information

Plasmid name: pLV3-CMV-PKN3(human)-3×HA-Puro
Alias: NM_013355.5;UTDP4-1
Gene lenght: 2667
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PKN3-3×HA-Puro plasmid Description

pLV3-CMV-PKN3-3×HA-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-PKN3-3×HA-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggaggag ggggcgccgc ggcagcctgg gccgagccag 2341 tggcccccag aggatgagaa ggaggtgatc cgccgggcca tccagaaaga gctgaagatc 2401 aaggaggggg tggagaacct gcggcgcgtg gccacagacc gccgccactt gggccatgtg 2461 cagcagctgc tgcggtcctc caaccgccgc ctggagcagc tgcatggcga gctgcgggag 2521 ctgcacgccc gaatcctgct gcccggccct gggcctggcc cagctgagcc tgtggcctca 2581 ggaccccggc cgtgggcaga gcagctcagg gctcggcacc tagaggctct ccggaggcag 2641 ctgcatgtgg agctgaaggt gaagcagggg gctgagaaca tgacccacac gtgcgccagt 2701 ggcaccccca aggagaggaa gctcctggca gctgcccagc agatgctgcg ggacagccag 2761 ctgaaggtgg ccctgctgcg gatgaagatc agcagcctgg aggccagtgg gtccccggag 2821 ccagggcctg agctgctggc ggaggagcta cagcatcgac tgcacgttga ggcagctgtg 2881 gctgagggcg ccaagaacgt ggtgaaactg cttagtagcc ggagaacaca ggaccgcaag 2941 gcactggctg aggcccaggc ccagctacag gagtcctctc agaaactgga cctcctgcgc 3001 ctggccttgg agcagctgct ggagcaactg cctcctgccc accctttgcg cagcagagtg 3061 acccgagagt tgcgggctgc ggtgcctgga tacccccagc cttcagggac acctgtgaag 3121 cccaccgccc taacagggac actgcaggtc cgcctcctgg gctgtgaaca gttgctgaca 3181 gccgtgcctg ggcgctcccc agcggccgca ctggccagca gcccctccga gggctggctt 3241 cggaccaagg ccaagcacca gcgtggccga ggcgagcttg ccagcgaggt gctggctgtg 3301 ctaaaggtgg acaaccgtgt tgtggggcag acgggctggg ggcaggtggc cgaacagtcc 3361 tgggaccaga cctttgtcat cccactggag cgagcccgtg agctggagat tggggtacac 3421 tggcgggact ggcggcagct atgtggcgtg gccttcctga gacttgagga cttcctggac 3481 aatgcctgtc accaactgtc cctcagcctg gtaccgcagg gactgctttt tgcccaggtg 3541 accttctgcg atcctgtcat tgagaggcgg ccccggctgc agaggcagga acgcatcttc 3601 tctaaacgca gaggccagga cttcctgagg gcttcgcaga tgaacctcgg catggcggcc 3661 tgggggcgcc tcgtcatgaa cctgctgccc ccctgcagct ccccgagcac aatcagcccc 3721 cctaaaggat gccctcggac cccaacaaca ctgcgagagg cctctgaccc tgccactccc 3781 agtaatttcc tgcccaagaa gacccccttg ggtgaagaga tgacaccccc acccaagccc 3841 ccacgcctct acctccccca ggagccaaca tccgaggaga ctccgcgcac caaacgtccc 3901 catatggagc ctaggactcg acgtgggcca tctccaccag cctcccccac caggaaaccc 3961 cctcggcttc aggacttccg ctgcttagct gtgctgggcc ggggacactt tgggaaggtc 4021 ctcctggtcc agttcaaggg gacagggaaa tactacgcca tcaaagcact gaagaagcag 4081 gaggtgctca gccgggacga gatagagagc ctgtactgcg agaagcggat cctggaggct 4141 gtgggctgca cagggcaccc tttcctgctc tccctccttg cctgcttcca gacctccagc 4201 catgcctgct ttgtgactga gtttgtgcct ggtggtgacc tcatgatgca gatccacgag 4261 gatgtcttcc ccgagcccca ggcccgcttc tacgtggctt gtgttgtcct ggggctgcag 4321 ttcttacacg agaagaagat catttacagg gacctgaagt tggataacct tctgctggat 4381 gcccagggat tcctgaagat cgcagacttt ggactctgca aggaagggat cggcttcggg 4441 gaccggacta gcaccttctg tggcaccccg gagttcctgg ctcccgaggt gctgacccag 4501 gaggcataca cacgggctgt ggactggtgg gggctgggtg tgctgctcta cgagatgctg 4561 gtgggtgagt gcccgttccc aggggacaca gaggaagagg tgtttgactg catcgtcaac 4621 atggacgccc cctaccccgg ctttctgtcg gtgcaagggc ttgagttcat tcagaagctc 4681 ctccagaagt gcccggagaa gcgcctcggg gcaggtgagc aggatgccga ggagatcaag 4741 gtccagccat tcttcaggac caccaactgg caagccctgc tcgcccgcac catccagccc 4801 cccttcgtgc ctaccctgtg tggccctgcg gacctgcgct actttgaggg cgagttcaca 4861 gggctgccgc ctgccctgac cccacctgca ccccacagcc tcctcactgc ccgccaacag 4921 gccgccttcc gggacttcga ctttgtgtca gagcgattcc tggaacccGG ATCCTACCCA 4981 TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT 5041 TATGATGTCC CAGACTACGC ATAGGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG 5101 TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA 5161 TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG 5221 GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA 5281 CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT 5341 CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT 5401 TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA 5461 GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG 5521 GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT 5581 TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGATGACC GAGTACAAGC 5641 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG 5701 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG 5761 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG 5821 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG 5881 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC 5941 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG 6001 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC 6061 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC 6121 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG 6181 AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGTCGAC AATCAACCTC 6241 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 6301 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 6361 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 6421 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 6481 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 6541 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 6601 ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG 6661 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 6721 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC 6781 CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGTACCTTT AAGACCAATG 6841 ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG 6901 CTAATTCACT CCCAACGAAA ATAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA 6961 GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA 7021 TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC 7081 TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT 7141 CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC 7201 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 7261 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT 7321 CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC 7381 ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT 7441 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGACTT TTGCAGAGAC GGCCCAAATT 7501 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 7561 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 7621 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 7681 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 7741 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 7801 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 7861 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 7921 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 7981 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 8041 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 8101 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 8161 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 8221 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 8281 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 8341 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 8401 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 8461 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 8521 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 8581 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 8641 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 8701 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 8761 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 8821 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 8881 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 8941 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 9001 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 9061 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 9121 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 9181 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 9241 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 9301 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 9361 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 9421 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 9481 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 9541 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 9601 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 9661 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA 9721 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 9781 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 9841 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 9901 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 9961 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG 10021 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG 10081 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.