pLV3-CMV-INTS7-3×FLAG-CopGFP-P

pLV3-CMV-INTS7-3×FLAG-CopGFP-Puro plasmid

PVT110090

pLV3-CMV-INTS7-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-INTS7(human)-3×FLAG-CopGFP-Puro
Alias: NM_015434.4;C1orf73; INT7
Gene lenght: 2886
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-INTS7-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-INTS7-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-INTS7-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg cgtcaaactc aactaagtct 2341 ttcctggcag atgccggcta tggcgaacag gaactggatg ccaactctgc ccttatggaa 2401 ttggacaaag gcctaagatc tggcaaactt ggtgaacagt gtgaagcagt tgttcgcttt 2461 cccagacttt ttcagaagta tccattccct attcttatca attctgcatt cctaaagtta 2521 gctgatgttt tcagagttgg aaataatttc ctgaggctat gtgttcttaa agttacccaa 2581 caaagtgaga aacatttgga gaagattcta aatgtggatg aatttgtgaa gagaattttt 2641 tctgtgattc atagtaatga tcctgtggca agagccatca ccctccggat gttgggaagt 2701 ctggcatcaa taattcctga gaggaagaat gctcatcata gtattcgtca gagtttagat 2761 tcacatgata atgtagaagt tgaagctgct gtttttgctg ctgcaaactt ctctgcacag 2821 tcaaaggatt ttgctgtagg aatctgtaac aaaatcagtg aaatgattca aggtttagcg 2881 acaccagtag acttgaagct aaaattgata cccattctac agcacatgca ccatgatgca 2941 atcttggctt ccagtgctcg tcagctttta caacagctgg tcacatccta tccgtccacc 3001 aaaatggtga ttgtgtcttt gcacactttc actctgcttg cagcgtcatc tttggttgat 3061 acacctaagc agattcagct tctgttgcag tatttgaaga atgatcccag gaaggcagta 3121 aagagacttg ctattcaaga tctgaaatta cttgctaata aaacaccaca tacttggagt 3181 agggagaata ttcaggcact ttgtgagtgt gccctccaga ctccttatga cagcttaaaa 3241 ctagggatgt tgtctgtcct ttccacacta tcagggacca tcgccatcaa acattacttc 3301 agtatagttc caggaaatgt gagttcttct cccagatctt ctgatttagt caaattagcc 3361 caagagtgct gttaccataa taacaggggc attgcagctc atggagttag agtcctaact 3421 aatataactg tttcttgtca agaaaaggat cttttggcac tggaacaaga tgctgtcttt 3481 ggcctggaat ccctactggt actttgtagt caagatgata gtccaggtgc tcaggccact 3541 ttaaagattg ctctaaactg tatggtgaag ttggccaagg gcaggcccca tcttagccag 3601 tcagtagttg agaccttgtt gactcaattg cacagtgctc aagacgctgc ccggattttg 3661 atgtgccatt gcctggcagc cattgccatg caactgccgg tgctgggtga tgggatgctt 3721 ggtgacctca tggagctgta caaggtgatt ggacgatcag ccacagacaa gcaacaagaa 3781 cttctggtga gtttggctac tgtgattttt gttgcaagtc agaaggcatt gtctgtggaa 3841 agtaaggcag taattaagca gcagcttgaa agtgtctcca atggatggac tgtataccgt 3901 attgccagac aggcttccag aatgggtaat catgacatgg ccaaagagct ttatcagagt 3961 ttgctgactc aggttgcctc agaacatttc tacttctggc taaatagttt gaaggagttt 4021 tcacatgcag aacagtgtct cactgggttg caagaggaaa attatagttc agcactttct 4081 tgcattgctg aatctttaaa attctatcac aaagggattg cttccttaac agcagctagt 4141 acaccactga atcctttaag ctttcagtgt gaatttgtaa aactcaggat tgacctttta 4201 caagccttct ctcaacttat ctgtacttgt aatagcctga agacaagccc accacctgca 4261 attgccacaa caattgccat gaccttagga aatgacctcc agaggtgtgg tcgcatctcc 4321 aatcagatga aacagtccat ggaagaattt cgaagccttg cttctcgata tggagatctt 4381 taccaggcat cttttgatgc tgactcagca actttgagga atgttgaact acagcagcag 4441 agctgtttac tgatatctca tgcaatagaa gccctgattt tggatccaga atcagcaagt 4501 ttccaggaat atggatctac tggaacagcc catgctgata gtgaatatga aagaagaatg 4561 atgtctgtat ataatcatgt cttggaggag gtagaatcac tcaatcggaa atatacccct 4621 gtttcttata tgcacacagc atgcctctgc aatgccatca ttgctttgct gaaagttccc 4681 ctttctttcc agagatattt tttccagaaa ctacagtcta ccagcatcaa gcttgctctg 4741 tcaccatcgc cccggaatcc tgcagagccc attgctgtcc agaataacca gcagctggcg 4801 ctaaaggtag agggagtggt tcagcacgga tctaaaccag gactcttccg caaaattcag 4861 tctgtctgtc tgaatgtttc ttccacactg cagagtaaat ctggacaaga ctacaagata 4921 cccattgaca acatgaccaa tgagatggag caaagggttg aacctcataa tgattacttc 4981 agtactcaat ttctgttgaa ctttgctatc cttggaacac acaacattac agtggaatct 5041 tctgtgaaag atgccaatgg tatagtatgg aagactggtc ccagaactac catatttgta 5101 aaatccctgg aagaccctta ttcccagcaa attcgcttac aacagcagca agcccagcag 5161 ccattacagc agcagcagca acgcaatgcc tacacacggt ttggatccGA CTACAAAGAC 5221 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 5281 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 5341 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 5401 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 5461 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 5521 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 5581 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 5641 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 5701 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 5761 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 5821 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 5881 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 5941 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 6001 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 6061 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 6121 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 6181 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 6241 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 6301 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 6361 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 6421 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 6481 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 6541 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 6601 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 6661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 6721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 6781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 6841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 6901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 6961 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 7021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 7081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 7141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 7201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 7261 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 7321 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 7381 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 7441 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 7501 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 7561 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 7621 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 7681 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 7741 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 7801 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 7861 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 7921 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 7981 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 8041 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 8101 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 8161 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 8221 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 8281 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 8341 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 8401 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 8461 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 8521 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 8581 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 8641 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 8701 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 8761 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 8821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 8881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 8941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 9001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 9061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 9121 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 9181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 9241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 9301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 9361 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 9421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 9481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 9541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 9601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 9661 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 9721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 9781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 9841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 9901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 9961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 10021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 10081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 10141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 10201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 10261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 10321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 10381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 10441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 10501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 10561 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 10621 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 10681 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 10741 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 10801 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 10861 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 10921 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 10981 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 11041 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 11101 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 11161 ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.