pLV2-TRE3GS-Tbx21-3×FLAG-T2A-E

pLV2-TRE3GS-Tbx21-3×FLAG-T2A-EGFP-TetOne-Puro plasmid

PVT110078

pLV2-TRE3GS-Tbx21-3×FLAG-T2A-EGFP-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-Tbx21(mouse)-3×FLAG-T2A-EGFP-TetOne-Puro
Alias: NM_019507.2;Tbet; Tblym; TBT1
Gene lenght: 1590
Host: Mammalian cells,lentivirus
Use(s): Gene expression,Tetracycline induction
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-Tbx21-3×FLAG-T2A-EGFP-TetOne-Puro plasmid Description

pLV2-TRE3GS-Tbx21-3×FLAG-T2A-EGFP-TetOne-Puro Plasmid suitable for gene expression in mammalian cells, tetracycline-inducible, also suitable for lentivirus packaging.

pLV2-TRE3GS-Tbx21-3×FLAG-T2A-EGFP-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac cATGGGCATC 6721 GTGGAGCCGG GCTGCGGAGA CATGCTGACC GGCACCGAGC CGATGCCGAG TGACGAGGGC 6781 CGGGGGCCCG GAGCGGACCA ACAGCATCGT TTCTTCTATC CCGAGCCGGG CGCACAGGAC 6841 CCGACCGATC GCCGCGCAGG TAGCAGCCTG GGGACGCCCT ACTCTGGGGG CGCCCTGGTG 6901 CCTGCCGCGC CGGGTCGCTT CCTTGGATCC TTCGCCTACC CGCCCCGGGC TCAGGTGGCT 6961 GGCTTTCCCG GGCCTGGCGA GTTCTTCCCG CCGCCCGCGG GTGCGGAGGG CTACCCGCCC 7021 GTGGATGGCT ACCCTGCCCC TGACCCGCGC GCGGGGCTCT ACCCAGGGCC GCGCGAGGAC 7081 TACGCATTGC CCGCGGGGTT GGAGGTGTCT GGGAAGCTGA GAGTCGCGCT CAGCAACCAC 7141 CTGTTGTGGT CCAAGTTCAA CCAGCACCAG ACAGAGATGA TCATCACTAA GCAAGGACGG 7201 CGAATGTTCC CATTCCTGTC CTTCACCGTG GCCGGGCTGG AGCCCACAAG CCATTACAGG 7261 ATGTTTGTGG ATGTGGTCTT GGTGGACCAG CACCACTGGC GGTACCAGAG CGGCAAGTGG 7321 GTGCAGTGTG GAAAGGCAGA AGGCAGCATG CCAGGGAACC GCTTATATGT CCACCCAGAC 7381 TCCCCCAACA CCGGAGCCCA CTGGATGCGC CAGGAAGTTT CATTTGGGAA GCTAAAGCTC 7441 ACCAACAACA AGGGGGCTTC CAACAATGTG ACCCAGATGA TCGTCCTGCA GTCTCTCCAC 7501 AAGTACCAGC CCCGGCTGCA CATCGTGGAG GTGAATGATG GAGAGCCAGA GGCTGCCTGC 7561 AGTGCTTCTA ACACACACGT CTTTACTTTC CAAGAGACCC AGTTCATTGC AGTGACTGCC 7621 TACCAGAACG CAGAGATCAC TCAGCTGAAA ATCGACAACA ACCCCTTTGC CAAAGGATTC 7681 CGGGAGAACT TTGAGTCCAT GTACGCATCT GTTGATACGA GTGTCCCCTC GCCACCTGGA 7741 CCCAACTGTC AACTGCTTGG GGGAGACCCC TTCTCACCTC TTCTATCCAA CCAGTATCCT 7801 GTTCCCAGCC GTTTCTACCC CGACCTTCCA GGCCAGCCCA AGGATATGAT CTCACAGCCT 7861 TACTGGCTGG GGACACCTCG GGAACACAGT TATGAAGCGG AGTTCCGAGC TGTGAGCATG 7921 AAGCCCACAC TCCTACCCTC TGCCCCGGGG CCCACTGTGC CCTACTACCG GGGCCAAGAC 7981 GTCCTGGCGC CTGGAGCTGG TTGGCCCGTG GCCCCTCAAT ACCCGCCCAA GATGAGCCCA 8041 GCTGGCTGGT TCCGGCCCAT GCGAACTCTG CCCATGGACC CGGGCCTGGG ATCCTCAGAG 8101 GAACAGGGCT CCTCCCCCTC GCTGTGGCCT GAGGTCACCT CCCTCCAGCC GGAGCCCAGC 8161 GACTCAGGAC TAGGCGAAGG AGACACTAAG AGGAGGAGGA TATCCCCCTA TCCTTCCAGT 8221 GGCGACAGCT CCTCTCCCGC TGGGGCCCCT TCTCCTTTTG ATAAGGAAAC CGAAGGCCAG 8281 TTTTATAATT ATTTTCCCAA CACCGGTGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 8341 CATGACATCG ACTACAAGGA TGACGATGAC AAGggtggct cagagggcag aggaagtctt 8401 ctaacatgcg gtgacgtgga ggagaatccc ggccctatgg tgagcaaggg cgaggagctg 8461 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 8521 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 8581 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 8641 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 8701 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 8761 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 8821 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 8881 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 8941 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 9001 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 9061 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 9121 gggatcactc tcggcatgga cgagctgtac aagtagggat ccagaccacc tcccctgcga 9181 gctaagctgg acagccaatg acgggtaaga gagtgacatt tttcactaac ctaagacagg 9241 agggccgtca gagctactgc ctaatccaaa gacgggtaaa agtgataaaa atgtatcact 9301 ccaacctaag acaggcgcag cttccgaggg atttgagatc cagacatgat aagatacatt 9361 gatgagtttg gacaaaccaa aactagaatg cagtgaaaaa aatgccttat ttgtgaaatt 9421 tgtgatgcta ttgccttatt tgtaaccatt ataagctgca ataaacaagt taagtcgata 9481 aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt 9541 ttgtagttct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact 9601 gtacccccca atcccccctt ttcttttaaa ggcgataccg tcgagatccg ttcactaatc 9661 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg 9721 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc 9781 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca 9841 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg 9901 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt 9961 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg 10021 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag 10081 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta 10141 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt 10201 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac 10261 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc 10321 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct 10381 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt 10441 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga 10501 tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt 10561 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc 10621 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg 10681 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg 10741 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg 10801 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc 10861 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg 10921 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc 10981 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac 11041 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac 11101 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag 11161 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc 11221 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct 11281 ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa 11341 gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca 11401 ctctaacact tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct 11461 ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta 11521 gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc 11581 agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga 11641 gtgaattagc ccttcca

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.