pLV3-CMV-KHDRBS3-3×FLAG-CopGFP

pLV3-CMV-KHDRBS3-3×FLAG-CopGFP-Puro plasmid

PVT110057

pLV3-CMV-KHDRBS3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KHDRBS3(human)-3×FLAG-CopGFP-Puro
Alias: NM_006558.3;Etle; etoile; SALP; SLM-2; SLM2; T-STAR;TSTAR;
Gene lenght: 1038
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KHDRBS3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-KHDRBS3-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-KHDRBS3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg aggagaagta cctgcccgag 2341 ctgatggcgg agaaggactc cctggacccc tccttcacgc acgccctgcg cctggtgaac 2401 caagaaatag aaaagtttca aaaaggagaa ggcaaggatg aagaaaagta catcgatgtg 2461 gtgattaata agaacatgaa gctgggacag aaagtgttaa ttcccgtaaa acagttccct 2521 aagttcaact ttgtggggaa acttttgggt ccacgtggca attctctgaa gcgtttacaa 2581 gaagaaacct tgacaaaaat gtccatcctt gggaaaggtt ccatgagaga caaggccaag 2641 gaagaagagt tgaggaaaag tggagaagcg aagtacttcc atctcaatga tgatctccat 2701 gttctcattg aagtgtttgc cccacctgca gaagcttatg ccaggatggg acatgctttg 2761 gaagaaatca aaaagttcct catccctgat tataatgatg agatcaggca agcacagctc 2821 caggagttaa catatttgaa tggtggttca gaaaatgcag atgttccagt ggttcgaggg 2881 aaacccacct tgcgtacaag aggtgtacca gccccagcaa taaccagggg aaggggagga 2941 gttacagccc ggccagttgg agttgtagta ccacgaggga cgccaactcc cagaggagtc 3001 ctgtccaccc gagggccagt gagtcgggga agaggacttc tcactcccag agcaagagga 3061 gtccccccaa ctgggtacag acctccaccg ccacccccga cacaagagac ttatggagaa 3121 tatgactatg atgatggata tggcactgct tatgatgaac agagttatga ttcctatgat 3181 aacagctata gcaccccagc ccaaagtggt gctgattact atgattacgg acatggactc 3241 agtgaggaga cttatgattc ctacgggcaa gaagagtgga ctaactcaag acacaaggca 3301 ccttcagcga ggacagcaaa gggcgtctac agagaccagc catatggcag atacggatcc 3361 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 3421 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 3481 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 3541 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 3601 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 3661 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 3721 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 3781 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 3841 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 3901 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 3961 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4021 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4081 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4141 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4201 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4261 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4321 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4381 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4441 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4501 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 4561 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 4621 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 4681 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 4741 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 4801 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 4861 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 4921 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 4981 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5041 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5101 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5161 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5221 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5281 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5341 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5401 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 5461 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 5521 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 5581 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 5641 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 5701 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 5761 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 5821 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 5881 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 5941 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6001 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6061 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 6121 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6181 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6241 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6301 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6361 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6421 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6481 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 6541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 6601 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 6661 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 6721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6781 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6841 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6901 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6961 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7081 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7981 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8881 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 8941 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9001 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9061 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9121 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9301 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.