pT7-6×His-SUMO-EGFP-HSP90AA1-6

pT7-6×His-SUMO-EGFP-HSP90AA1-6×His plasmid

PVT110005

pT7-6×His-SUMO-EGFP-HSP90AA1-6×His plasmid Information

Plasmid name: pT7-6×His-SUMO-EGFP-HSP90AA1(human)-6×His
Alias: NM_001017963.3;EL52; HEL-S-65p; Hsp103; HSP86; Hsp89;HSP89A; Hsp90; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1;HSPCAL4; HSPN; LAP-2; LAP2
Gene lenght: 2562
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-6×His-SUMO-EGFP-HSP90AA1-6×His plasmid Description

pT7-6×His-SUMO-EGFP-HSP90AA1-6×His Plasmid suitable for gene expression in Escherichia coli.

pT7-6×His-SUMO-EGFP-HSP90AA1-6×His plasmid Sequence

1 CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA 61 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA 181 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG 241 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGG 301 GCAGCAGCCA TCATCATCAT CATCACGGCA GCGGCCTGGT GCCGCGCGGC AGCGCTAGCA 361 TGTCGGACTC AGAAGTCAAT CAAGAAGCTA AGCCAGAGGT CAAGCCAGAA GTCAAGCCTG 421 AGACTCACAT CAATTTAAAG GTGTCCGATG GATCTTCAGA GATCTTCTTC AAGATCAAAA 481 AGACCACTCC TTTAAGAAGG CTGATGGAAG CGTTCGCTAA AAGACAGGGT AAGGAAATGG 541 ACTCCTTAAG ATTCTTGTAC GACGGTATTA GAATTCAAGC TGATCAGACC CCTGAAGATT 601 TGGACATGGA GGATAACGAT ATTATTGAGG CTCACAGAGA ACAGATTGGT GGTAGACAAG 661 CTatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 721 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 781 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 841 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 901 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 961 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 1021 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 1081 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 1141 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 1201 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 1261 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 1321 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg 1381 gcggtggagg cagtggtgga ggcggtagca tgcccccgtg ttcgggcggg gacggctcca 1441 cccctcctgg gccctccctt cgggacaggg actgtcccgc ccagagtgct gaatacccgc 1501 gcgaccgtct ggatccccgc ccaggaagcc cctctgaagc ctcctcgccg ccgtttctga 1561 gaagcagggc acctgttaac tggtaccaag aaaaggccca agtgtttctc tggcatctga 1621 tggtgtctgg atccaccact ctactctgtc tctggaaaca gcccttccac gtctctgcat 1681 tccctgtcac cgcgtcactg gccttcagac agagccaagg tgcagggcaa cacctctaca 1741 aggatctgca gccatttata ttgcttaggc tactgatgcc tgaggaaacc cagacccaag 1801 accaaccgat ggaggaggag gaggttgaga cgttcgcctt tcaggcagaa attgcccagt 1861 tgatgtcatt gatcatcaat actttctact cgaacaaaga gatctttctg agagagctca 1921 tttcaaattc atcagatgca ttggacaaaa tccggtatga aagcttgaca gatcccagta 1981 aattagactc tgggaaagag ctgcatatta accttatacc gaacaaacaa gatcgaactc 2041 tcactattgt ggatactgga attggaatga ccaaggctga cttgatcaat aaccttggta 2101 ctatcgccaa gtctgggacc aaagcgttca tggaagcttt gcaggctggt gcagatatct 2161 ctatgattgg ccagttcggt gttggttttt attctgctta tttggttgct gagaaagtaa 2221 ctgtgatcac caaacataac gatgatgagc agtacgcttg ggagtcctca gcagggggat 2281 cattcacagt gaggacagac acaggtgaac ctatgggtcg tggaacaaaa gttatcctac 2341 acctgaaaga agaccaaact gagtacttgg aggaacgaag aataaaggag attgtgaaga 2401 aacattctca gtttattgga tatcccatta ctctttttgt ggagaaggaa cgtgataaag 2461 aagtaagcga tgatgaggct gaagaaaagg aagacaaaga agaagaaaaa gaaaaagaag 2521 agaaagagtc ggaagacaaa cctgaaattg aagatgttgg ttctgatgag gaagaagaaa 2581 agaaggatgg tgacaagaag aagaagaaga agattaagga aaagtacatc gatcaagaag 2641 agctcaacaa aacaaagccc atctggacca gaaatcccga cgatattact aatgaggagt 2701 acggagaatt ctataagagc ttgaccaatg actgggaaga tcacttggca gtgaagcatt 2761 tttcagttga aggacagttg gaattcagag cccttctatt tgtcccacga cgtgctcctt 2821 ttgatctgtt tgaaaacaga aagaaaaaga acaacatcaa attgtatgta cgcagagttt 2881 tcatcatgga taactgtgag gagctaatcc ctgaatatct gaacttcatt agaggggtgg 2941 tagactcgga ggatctccct ctaaacatat cccgtgagat gttgcaacaa agcaaaattt 3001 tgaaagttat caggaagaat ttggtcaaaa aatgcttaga actctttact gaactggcgg 3061 aagataaaga gaactacaag aaattctatg agcagttctc taaaaacata aagcttggaa 3121 tacacgaaga ctctcaaaat cggaagaagc tttcagagct gttaaggtac tacacatctg 3181 cctctggtga tgagatggtt tctctcaagg actactgcac cagaatgaag gagaaccaga 3241 aacatatcta ttatatcaca ggtgagacca aggaccaggt agctaactca gcctttgtgg 3301 aacgtcttcg gaaacatggc ttagaagtga tctatatgat tgagcccatt gatgagtact 3361 gtgtccaaca gctgaaggaa tttgagggga agactttagt gtcagtcacc aaagaaggcc 3421 tggaacttcc agaggatgaa gaagagaaaa agaagcagga agagaaaaaa acaaagtttg 3481 agaacctctg caaaatcatg aaagacatat tggagaaaaa agttgaaaag gtggttgtgt 3541 caaaccgatt ggtgacatct ccatgctgta ttgtcacaag cacatatggc tggacagcaa 3601 acatggagag aatcatgaaa gctcaagccc taagagacaa ctcaacaatg ggttacatgg 3661 cagcaaagaa acacctggag ataaaccctg accattccat tattgagacc ttaaggcaaa 3721 aggcagaggc tgataagaac gacaagtctg tgaaggatct ggtcatcttg ctttatgaaa 3781 ctgcgctcct gtcttctggc ttcagtctgg aagatcccca gacacatgct aacaggatct 3841 acaggatgat caaacttggt ctgggtattg atgaagatga ccctactgct gatgatacca 3901 gtgctgctgt aactgaagaa atgccacccc ttgaaggaga tgacgacaca tcacgcatgg 3961 aagaagtaga ccTCGAGCAC CACCACCACC ACCACTGAGA TCCGGCTGCT AACAAAGCCC 4021 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG 4081 CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATTGGCGA 4141 ATGGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT 4201 GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT 4261 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG 4321 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG 4381 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 4441 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 4501 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 4561 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAGGTGGCAC TTTTCGGGGA 4621 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC 4681 ATGAATTAAT TCTTAGAAAA ACTCATCGAG CATCAAATGA AACTGCAATT TATTCATATC 4741 AGGATTATCA ATACCATATT TTTGAAAAAG CCGTTTCTGT AATGAAGGAG AAAACTCACC 4801 GAGGCAGTTC CATAGGATGG CAAGATCCTG GTATCGGTCT GCGATTCCGA CTCGTCCAAC 4861 ATCAATACAA CCTATTAATT TCCCCTCGTC AAAAATAAGG TTATCAAGTG AGAAATCACC 4921 ATGAGTGACG ACTGAATCCG GTGAGAATGG CAAAAGTTTA TGCATTTCTT TCCAGACTTG 4981 TTCAACAGGC CAGCCATTAC GCTCGTCATC AAAATCACTC GCATCAACCA AACCGTTATT 5041 CATTCGTGAT TGCGCCTGAG CGAGACGAAA TACGCGATCG CTGTTAAAAG GACAATTACA 5101 AACAGGAATC GAATGCAACC GGCGCAGGAA CACTGCCAGC GCATCAACAA TATTTTCACC 5161 TGAATCAGGA TATTCTTCTA ATACCTGGAA TGCTGTTTTC CCGGGGATCG CAGTGGTGAG 5221 TAACCATGCA TCATCAGGAG TACGGATAAA ATGCTTGATG GTCGGAAGAG GCATAAATTC 5281 CGTCAGCCAG TTTAGTCTGA CCATCTCATC TGTAACATCA TTGGCAACGC TACCTTTGCC 5341 ATGTTTCAGA AACAACTCTG GCGCATCGGG CTTCCCATAC AATCGATAGA TTGTCGCACC 5401 TGATTGCCCG ACATTATCGC GAGCCCATTT ATACCCATAT AAATCAGCAT CCATGTTGGA 5461 ATTTAATCGC GGCCTAGAGC AAGACGTTTC CCGTTGAATA TGGCTCATAA CACCCCTTGT 5521 ATTACTGTTT ATGTAAGCAG ACAGTTTTAT TGTTCATGAC CAAAATCCCT TAACGTGAGT 5581 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 5641 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 5701 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 5761 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 5821 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 5881 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 5941 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 6001 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6061 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6121 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6181 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6241 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 6301 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 6361 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC 6421 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCAATGGTGC ACTCTCAGTA CAATCTGCTC 6481 TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT 6541 GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 6601 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 6661 TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT 6721 TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT 6781 GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT 6841 GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG 6901 ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT 6961 AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG 7021 CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG 7081 ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG 7141 AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT 7201 CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT 7261 AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG 7321 ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG 7381 AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA 7441 AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG 7501 ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG 7561 CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC 7621 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC 7681 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG 7741 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC 7801 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT 7861 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC 7921 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC 7981 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT 8041 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT 8101 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT 8161 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC 8221 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC 8281 ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC 8341 ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC 8401 CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG 8461 TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT 8521 GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT 8581 GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC 8641 GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC 8701 GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG 8761 CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT 8821 CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA 8881 CCGCCGCCGC AAGGAATGGT GCATG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.