pCMV-Xiap-3×FLAG-T2A-EGFP-Neo

pCMV-Xiap-3×FLAG-T2A-EGFP-Neo plasmid

PVT109887

pCMV-Xiap-3×FLAG-T2A-EGFP-Neo plasmid Information

Plasmid name: pCMV-Xiap(mouse)-3×FLAG-T2A-EGFP-Neo
Alias: NM_001301639.1;1110015C02Rik; Aipa; Api3; Birc4; IAP3;ILP-1; MIHA
Gene lenght: 783
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Xiap-3×FLAG-T2A-EGFP-Neo plasmid Description

pCMV-Xiap-3×FLAG-T2A-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Xiap-3×FLAG-T2A-EGFP-Neo plasmid Sequence

1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat 61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc 841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag 901 cgtttaaact taagcttGCC ACCatgactt ttaacagttt tgaaggaact agaacttttg 961 tacttgcaga caccaataag gatgaagaat ttgtagaaga gtttaataga ttaaaaacat 1021 ttgctaactt cccaagtagt agtcctgttt cagcatcaac attggcgcga gctgggtttc 1081 tttataccgg tgaaggagac accgtgcaat gtttcagttg tcatgcggca atagatagat 1141 ggcagtatgg agactcagct gttggaagac acaggagaat atccccaaat tgcagattta 1201 tcaatggttt ttattttgaa aatggtgctg cacagtctac aaatcctggt atccaaaatg 1261 gccagtacaa atctgaaaac tgtgtgggaa atagaaatcc ttttgcccct gacaggccac 1321 ctgagactca tgctgattat ctcttgagaa ctggacaggt tgtagatatt tcagacacca 1381 tatacccgag gaaccctgcc atgtgtagtg aagaagccag attgaagtca tttcagaact 1441 ggccggacta tgctcattta acccccagag agttagctag tgctggcctc tactacacag 1501 gggctgatga tcaagtgcaa tgcttttgtt gtgggggaaa actgaaaaat tgggaaccct 1561 gtgatcgtgc ctggtcagaa cacaggagac actttcccaa ttgctttttt gttttgggcc 1621 ggaacgttaa tgttcgaagt gaatctggtg tgagttctga taggaatttc ccaaattcaa 1681 caaactctcc aagaaatcca gccatggcag aatatgaagc acggatcgtt acttttggaa 1741 catggacatc ctcagttaac aaggagcagc ttgcaagagc tggattttat gctttaggtg 1801 aaggcgataa agtgaagtgc tttcactgtg gaggagggct cacggattgg aagccaagtg 1861 aagacccttg ggaacagcat gcgaagtggt acccagggtg caaataccta ttggatgaga 1921 aggggcaaga atatataaat aatattcatt taacccattc acttgaggaa tctttgggaa 1981 gaactgctga aaaaacacca tcgctaacta aaaaaatcga tgataccatc ttccagaatc 2041 ctatggtgca agaagctata cgaatgggat ttagcttcaa ggacattaag aaaacaatgg 2101 aagaaaaaat ccaaacatcc gggagcagct atctatcact tgaggtcctg attgcagatc 2161 ttgtgagtgc tcagaaagat aatacggagg atgagtcaag tcaaacttca ttgcagaaag 2221 acattagtac tgaagagcag ctaaggcgcc tacaagagga gaagctttgc aaaatctgta 2281 tggatagaaa tattgctatc gtttttgttc cttgtggaca tctggtcact tgtaaacagt 2341 gtgcagaagc agttgacaaa tgtcccatgt gctacaccgt cattacgttc aagcaaaaaa 2401 tttttatgtc tgaattcatg GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA 2461 TCGACTACAA GGATGACGAT GACAAGccgc gggagggcag aggaagtctg ctaacatgcg 2521 gtgacgtcga ggagaatcct ggcccagccc gggatccacc ggtcgccacc atggtgagca 2581 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 2641 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 2701 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 2761 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 2821 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 2881 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 2941 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 3001 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 3061 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 3121 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 3181 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 3241 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgct 3301 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 3361 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 3421 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 3481 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 3541 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 3601 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 3661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 3721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 3781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 3841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 3901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 3961 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 4021 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 4081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 4141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4201 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 4261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 4321 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 4381 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 4441 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 4501 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 4561 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 4621 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 4681 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 4741 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 4801 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 4861 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 4921 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 4981 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 5041 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 5101 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 5161 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 5221 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 5281 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 5341 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 5401 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 5461 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 5521 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 5581 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 5641 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 5701 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 5761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 5821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 5881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 5941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 6001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 6121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 6181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 6241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 6301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 6361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 6421 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 6481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca 6541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 7081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 7381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7741 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.